ARFGAP1 plays a central role in coupling COPI cargo sorting with vesicle formation.


Journal Article

Examining how key components of coat protein I (COPI) transport participate in cargo sorting, we find that, instead of ADP ribosylation factor 1 (ARF1), its GTPase-activating protein (GAP) plays a direct role in promoting the binding of cargo proteins by coatomer (the core COPI complex). Activated ARF1 binds selectively to SNARE cargo proteins, with this binding likely to represent at least a mechanism by which activated ARF1 is stabilized on Golgi membrane to propagate its effector functions. We also find that the GAP catalytic activity plays a critical role in the formation of COPI vesicles from Golgi membrane, in contrast to the prevailing view that this activity antagonizes vesicle formation. Together, these findings indicate that GAP plays a central role in coupling cargo sorting and vesicle formation, with implications for simplifying models to describe how these two processes are coupled during COPI transport.

Full Text

Cited Authors

  • Lee, SY; Yang, J-S; Hong, W; Premont, RT; Hsu, VW

Published Date

  • January 2005

Published In

Volume / Issue

  • 168 / 2

Start / End Page

  • 281 - 290

PubMed ID

  • 15657398

Pubmed Central ID

  • 15657398

Electronic International Standard Serial Number (EISSN)

  • 1540-8140

International Standard Serial Number (ISSN)

  • 0021-9525

Digital Object Identifier (DOI)

  • 10.1083/jcb.200404008


  • eng