Induction of cytotoxic T cell responses in newborn mice by DNA immunization.
Published
Journal Article
Cytotoxic T cells (CTL) play a critical role in controlling viral infections. Infection of neonatal NFSIN mice with a high dose of Cas-Br-M murine leukemia virus, a neuropathogenic type C retrovirus, results in virus-induced neurologic disease and in their failure to generate a protective CTL response. Cas-Br-M-specific CTL are necessary in the protection of neonatal mice from Cas-Br-M-induced neurologic disease. Here we demonstrate that intramuscular inoculation of newborn mice with naked DNA expressing the full length Cas-Br-M genome induces a virus-specific CTL-mediated response. This CTL response is mediated by CD8+ T cells, is long lasting and, when transferred to susceptible neonatal recipients, protects them from Cas-induced neurologic disease. We also provide evidence that the intramuscular inoculation of neonates with plasmid DNA encoding only env sequences induces a dose-dependent CTL response in the absence of an anti-MuLV antibody response.
Full Text
Duke Authors
Cited Authors
- Sarzotti, M; Dean, TA; Remington, MP; Ly, CD; Furth, PA; Robbins, DS
Published Date
- June 1997
Published In
Volume / Issue
- 15 / 8
Start / End Page
- 795 - 797
PubMed ID
- 9234516
Pubmed Central ID
- 9234516
International Standard Serial Number (ISSN)
- 0264-410X
Digital Object Identifier (DOI)
- 10.1016/s0264-410x(96)00250-2
Language
- eng
Conference Location
- Netherlands