Treatment of intracranial alphavirus infections in mice by a combination of specific antibodies and an interferon inducer.

Published

Journal Article

Finding an effective treatment for viral infections that cause encephalitis remains an important problem. A model of human alphavirus infections, Semliki Forest virus, causes lethal encephalitis in weanling mice. Mice are viremic within 24 hr of an intraperitoneal challenge with the equivalent of three 75% lethal doses of Semliki Forest virus. Virus reaches the brain by 48 hr, and mortality results in all mice in 5-7 days. Introduction of virus intracranially accelerates the course of the infection. Neither anti-Semliki Forest virus hyperimmune serum nor the potent interferon inducer poly I:CLC given intraperitoneally are protective when used therapeutically after an intracranial virus infection, but a combination of 1,000 U hyperimmune serum and 80 micrograms/mouse of poly I:CLC results in a 50% survival rate. This combination treatment of intracranial Semliki Forest virus infection eliminates detectable viremia and reduces virus load in the brain over the course of the infection. These data show that when combined, specific antibody and an interferon inducer can interact synergistically to protect mice from alphavirus infections of the central nervous system even when given after the virus is replicating in the target organ.

Full Text

Duke Authors

Cited Authors

  • Coppenhaver, DH; Singh, IP; Sarzotti, M; Levy, HB; Baron, S

Published Date

  • January 1995

Published In

Volume / Issue

  • 52 / 1

Start / End Page

  • 34 - 40

PubMed ID

  • 7531956

Pubmed Central ID

  • 7531956

International Standard Serial Number (ISSN)

  • 0002-9637

Digital Object Identifier (DOI)

  • 10.4269/ajtmh.1995.52.34

Language

  • eng

Conference Location

  • United States