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Long-term killing of natural killer-resistant target cells by interferon-alpha-, interferon-gamma-, and interleukin-2-activated natural killer cells.

Publication ,  Journal Article
Sarzotti, M; Klimpel, GR; Baron, S
Published in: Nat Immun Cell Growth Regul
1989

The sensitivity of target cells to natural killer (NK) cell-mediated cytotoxicity was investigated. Five target cell lines were examined for susceptibility to killing by activated NK cells in a 4-hour cytotoxicity assay: one of them (K562) was highly sensitive, while the other four were resistant. However, the four NK-resistant target cell lines were fully susceptible to lysis when the assay was extended to 24 h. The cytotoxic cells that killed the NK-resistant target cells in a 24-hour assay were plastic- and nylon wool-nonadherent human peripheral blood mononuclear cells (PBMC) and their cytotoxicity was increased by interferon-alpha, interferon-gamma, and interleukin-2. Further, the cytotoxic activity of PBMC in the long-term assay was associated with large granular lymphocytes purified on a Percoll gradient, that killed the NK-sensitive cell line K562 in a 4-hour assay. All of the above are general criteria to qualify the cytotoxic cells as NK cells. Thus, the NK-resistant phenotype may not reflect absolute immunity to NK-mediated lysis, but it may reflect the different rates at which various target cell lines can be killed.

Duke Scholars

Published In

Nat Immun Cell Growth Regul

ISSN

0254-7600

Publication Date

1989

Volume

8

Issue

2

Start / End Page

66 / 75

Location

Switzerland

Related Subject Headings

  • Tumor Cells, Cultured
  • Time Factors
  • Recombinant Proteins
  • Leukocytes, Mononuclear
  • Killer Cells, Natural
  • Interleukin-2
  • Interferon-gamma
  • Interferon Type I
  • Cytotoxicity Tests, Immunologic
  • Cells, Cultured
 

Published In

Nat Immun Cell Growth Regul

ISSN

0254-7600

Publication Date

1989

Volume

8

Issue

2

Start / End Page

66 / 75

Location

Switzerland

Related Subject Headings

  • Tumor Cells, Cultured
  • Time Factors
  • Recombinant Proteins
  • Leukocytes, Mononuclear
  • Killer Cells, Natural
  • Interleukin-2
  • Interferon-gamma
  • Interferon Type I
  • Cytotoxicity Tests, Immunologic
  • Cells, Cultured