Interferon production in mixed cultures of murine leukocytes and syngeneic L1210 leukemia cells.


Journal Article

Spleen cells from DBA/2 mice did not proliferate, but released interferon (IFN) when cultured in the presence of mitomycin C-treated syngeneic L1210 leukemia cells apparently free from mycoplasma and common non-oncogenic viral infections. IFN titers reached a plateau after 18 h of culture. The biological activity of this IFN was stable at pH 2 and could be inactivated by antibody raised against alpha and beta-IFN. Removal of phagocytes from the spleen cell suspensions caused a decrease in IFN production. By contrast, suspensions depleted in Thy 1.2-positive cells had an enhanced capacity to produce IFN in response to the L1210 cells.

Full Text

Duke Authors

Cited Authors

  • Sarzotti, M; Giovarelli, M; Forni, G

Published Date

  • 1982

Published In

Volume / Issue

  • 61 / 3

Start / End Page

  • 218 - 224

PubMed ID

  • 6085676

Pubmed Central ID

  • 6085676

International Standard Serial Number (ISSN)

  • 0021-2547


  • eng

Conference Location

  • Italy