Effect of process parameters on production of a biopolymer by Rhizobium sp.


Journal Article (Academic article)

The production of biopolymers by a Rhizobium strain was studied under batch and bioreactor conditions. The best viscosity levels were obtained under low mannitol concentrations as well as low agitation and aeration conditions. Infrared spectra indicated the presence of chemical groups characteristic of microbially produced biopolymers, including C = O and O-acetyl groups. Thermogravimetric analysis showed the characteristic degradation profiles of the exopolysaccharide produced (Tonset = 290°C). The experimental design showed that a low substrate concentration (10.0 g/L), and low aeration (0.2 vvm) and agitation (200 rpm) levels should be used. The maximum yield of the process was a Yp/s (g/g) of 0.19 ± 0.1, obtained under optimized conditions.

Duke Authors

Cited Authors

  • Duta, FP; De Franca, FP; Servulo, EFC; De Almeida Lopes, LM; Da Costa, ACA; Barros, A

Published Date

  • 2004

Published In

  • Applied Biochemistry and Biotechnology Part a Enzyme Engineering and Biotechnology

Volume / Issue

  • 113-116 /

Start / End Page

  • 639 - 652