Membrane tethers formed from blood cells with available area and determination of their adhesion energy.

Published

Journal Article

Fundamental to all mammalian cells is the adherence of the lipid bilayer membrane to the underlying membrane associated cytoskeleton. To investigate this adhesion, we physically detach the lipid membrane from the cell by mechanically forming membrane tethers. For the most part these have been tethers formed from either neutrophils or red cells. Here we do a simple thermodynamic analysis of the tether formation process using the entire cell, including tether, as the control volume. For a neutrophil, we show that the total adhesion energy per unit area between lipid membrane and cytoskeleton depends on the square of the tether force. For a flaccid red cell, we show that the total adhesion energy minus the tension in the spectrin cytoskeleton depends also on the square of the tether force. Finally, we discuss briefly the viscous flow of membrane. Using published data we calculate and compare values for the various adhesion energies and viscosities.

Full Text

Duke Authors

Cited Authors

  • Hochmuth, RM; Marcus, WD

Published Date

  • June 2002

Published In

Volume / Issue

  • 82 / 6

Start / End Page

  • 2964 - 2969

PubMed ID

  • 12023219

Pubmed Central ID

  • 12023219

Electronic International Standard Serial Number (EISSN)

  • 1542-0086

International Standard Serial Number (ISSN)

  • 0006-3495

Digital Object Identifier (DOI)

  • 10.1016/s0006-3495(02)75637-3

Language

  • eng