Altered distribution of mitochondria and actin fibers in 3T3 cells cultured on microcarriers.

Journal Article (Journal Article)

The mitochondria and actin fibers of 3T3 fibroblasts cultured on microcarriers in spinner flasks were visualized using fluorescent stains. In contrast to cells grown on planar surfaces under static or steady laminar flow conditions, cells exposed to higher levels of turbulent agitation do not form actin stress fibers. Greater agitation also leads to a more diffuse appearance of the mitochondria and a wider distribution of them throughout the cytoplasm. This response may indicate damaged mitochondria, as similar results have been reported for chemical toxins.

Full Text

Duke Authors

Cited Authors

  • du Laney, TV; Cherry, RS; Coppinger, JA; Truskey, GA

Published Date

  • November 1992

Published In

Volume / Issue

  • 8 / 6

Start / End Page

  • 572 - 575

PubMed ID

  • 1282019

Electronic International Standard Serial Number (EISSN)

  • 1520-6033

International Standard Serial Number (ISSN)

  • 8756-7938

Digital Object Identifier (DOI)

  • 10.1021/bp00018a600


  • eng