Design of thermally responsive, recombinant polypeptide carriers for targeted drug delivery.

Journal Article (Review;Journal Article)

In this article, we review recombinant DNA methods for the design and synthesis of amino acid-based biopolymers, and briefly summarize an approach, recursive directional ligation (RDL), that we have employed to synthesize oligomeric genes for such biopolymers. We then describe our ongoing research in the use of RDL to synthesize recombinant polypeptide carriers for the targeted delivery of radionuclides, chemotherapeutics and biomolecular therapeutics to tumors. The targeted delivery system uses a thermally responsive, elastin-like polypeptide (ELP) as the drug carrier to enhance the localization of ELP-drug conjugates within a solid tumor that is heated by regional hyperthermia. In the context of this drug delivery application, we discuss the design of ELPs and their recombinant synthesis, which enables the molecular weight and the thermal properties of the polypeptide to be precisely controlled. Finally, our results pertaining to the in vivo targeting of tumors with ELPs are briefly summarized.

Full Text

Duke Authors

Cited Authors

  • Chilkoti, A; Dreher, MR; Meyer, DE

Published Date

  • October 2002

Published In

Volume / Issue

  • 54 / 8

Start / End Page

  • 1093 - 1111

PubMed ID

  • 12384309

Electronic International Standard Serial Number (EISSN)

  • 1872-8294

International Standard Serial Number (ISSN)

  • 0169-409X

Digital Object Identifier (DOI)

  • 10.1016/s0169-409x(02)00060-1


  • eng