Nitroxide conjugate of a thermally responsive elastin-like polypeptide for noninvasive thermometry.

Journal Article (Journal Article)

Hyperthermia, as an adjuvant with radiation and chemotherapy, has shown promise in the treatment of cancer. The relevant biological effects of a hyperthermia treatment are both time and temperature-dependent, creating a need for accurate thermometry. We present a novel noninvasive thermometry modality that combines a temperature responsive biopolymer, the elastin-like polypeptide (ELP), and nitroxide to produce an ELP-nitroxide conjugate. When examined with electron paramagnetic resonance (EPR) spectroscopy, the ELP-nitroxide conjugate has temperature-dependent spectral line widths whose predictive accuracy is approximately 0.3 degrees C (80 microM). We believe that the temperature-dependent changes observed in the EPR spectrum are due to the combined effect of temperature, viscosity and effective radius on the rotational correlation time of the ELP-nitroxide conjugate.

Full Text

Duke Authors

Cited Authors

  • Dreher, MR; Elas, M; Ichikawa, K; Barth, ED; Chilkoti, A; Rosen, GM; Halpern, HJ; Dewhirst, M

Published Date

  • October 2004

Published In

Volume / Issue

  • 31 / 10

Start / End Page

  • 2755 - 2762

PubMed ID

  • 15543780

International Standard Serial Number (ISSN)

  • 0094-2405

Digital Object Identifier (DOI)

  • 10.1118/1.1782677


  • eng

Conference Location

  • United States