Enzymatic fabrication of DNA nanostructures: extension of a self-assembled oligonucleotide monolayer on gold arrays.

Published

Journal Article

Nucleic acid nanostructures are useful as templates for bionanofabrication of composite molecular nanostructures in materials science, molecular electronics, and biosensing. Here, we demonstrate that terminal deoxynucleotidyl transferase, which repetitively adds mononucleotides to the 3' end of a short DNA initiator, can be used to rapidly fabricate DNA nanostructures up to 121 nm high with lateral dimensions from 0.1 to 4 mum in 2 h. These programmable scaffolds can potentially be employed to build more complex nanostructures consisting of natural or unnatural nucleotides with selective docking sites along the single-stranded DNA.

Full Text

Duke Authors

Cited Authors

  • Chow, DC; Lee, W-K; Zauscher, S; Chilkoti, A

Published Date

  • October 2005

Published In

Volume / Issue

  • 127 / 41

Start / End Page

  • 14122 - 14123

PubMed ID

  • 16218572

Pubmed Central ID

  • 16218572

Electronic International Standard Serial Number (EISSN)

  • 1520-5126

International Standard Serial Number (ISSN)

  • 0002-7863

Digital Object Identifier (DOI)

  • 10.1021/ja052491z

Language

  • eng