Protein-based molecular contrast optical coherence tomography with phytochrome as the contrast agent.

Journal Article (Journal Article)

We report the use of phytochrome A (phyA), a plant protein that can reversibly switch between two states with different absorption maxima (at 660 and 730 nm), as a contrast agent for molecular contrast optical coherence tomography (MCOCT). Our MCOCT scheme builds up a difference image revealing the distribution of phyA within a target sample from pairs of consecutive OCT A-scans acquired at a probe wavelength of 750 nm, both with and without additional illumination of the target sample with 660-nm light. We demonstrate molecular imaging with this new MCOCT modality in a target sample containing a mixture of 0.2% Intralipid and 83 microM of phyA.

Full Text

Duke Authors

Cited Authors

  • Yang, C; Choma, MA; Lamb, LE; Simon, JD; Izatt, JA

Published Date

  • June 2004

Published In

Volume / Issue

  • 29 / 12

Start / End Page

  • 1396 - 1398

PubMed ID

  • 15233447

Electronic International Standard Serial Number (EISSN)

  • 1539-4794

International Standard Serial Number (ISSN)

  • 0146-9592

Digital Object Identifier (DOI)

  • 10.1364/ol.29.001396


  • eng