Characterizing cellular contractility and cytoplasmic flow using spectral domain phase microscopy


Journal Article

The phase information inherent to spectral domain optical coherence tomography (OCT) processing can be exploited to resolve sub-coherence length displacement variations. Spectral domain phase microscopy (SDPM) is a functional extension of Fourier domain OCT whose common-path topology enables extraordinary phase sensitivity. Here we demonstrate the usefulness of SDPM in three biologically relevant applications: real-time tracking of cell surface displacements of contracting cardiac myocytes, extracting cytoplasmic flow characteristics for a single-celled organism (flow rates ∼10-30μm/s), and cellular mechanical responses to cytoskeletal drug treatments. The results of these experiments are corroborated by light microscopy acquired concurrently with the SDPM data.

Full Text

Duke Authors

Cited Authors

  • Ellerbee, AK; Choma, MA; McDowell, EJ; Creazzo, AL; Izatt, JA

Published Date

  • July 21, 2005

Published In

Volume / Issue

  • 5690 /

Start / End Page

  • 403 - 407

International Standard Serial Number (ISSN)

  • 1605-7422

Digital Object Identifier (DOI)

  • 10.1117/12.592809

Citation Source

  • Scopus