Doppler flow imaging of cytoplasmic streaming using spectral domain phase microscopy.

Published

Journal Article

Spectral domain phase microscopy (SDPM) is a function extension of spectral domain optical coherence tomography. SDPM achieves exquisite levels of phase stability by employing common-path interferometry. We discuss the theory and limitations of Doppler flow imaging using SDPM, demonstrate monitoring the thermal contraction of a glass sample with nanometer per second velocity sensitivity, and apply this technique to measurement of cytoplasmic streaming in an Amoeba proteus pseudopod. We observe reversal of cytoplasmic flow induced by extracellular CaCl2, and report results that suggest parabolic flow of cytoplasm in the A. proteus pseudopod.

Full Text

Duke Authors

Cited Authors

  • Choma, MA; Ellerbee, AK; Yazdanfar, S; Izatt, JA

Published Date

  • March 2006

Published In

Volume / Issue

  • 11 / 2

Start / End Page

  • 024014 -

PubMed ID

  • 16674204

Pubmed Central ID

  • 16674204

Electronic International Standard Serial Number (EISSN)

  • 1560-2281

International Standard Serial Number (ISSN)

  • 1083-3668

Digital Object Identifier (DOI)

  • 10.1117/1.2193167

Language

  • eng