Molecular contrast in optical coherence tomography using a pump-probe technique and a optical switch suppression technique


Journal Article

We describe two novel technique for contrast enhancement in optical coherence tomography (OCT) which enables molecular specific imaging. The first, a pump-probe technique, is employed in which a pulsed pump laser is tuned to ground-state absorption in a molecule of interest. The location of the target molecule population is derived from the resulting transient absorption of OCT sample arm light acting as probe light. Preliminary results exhibiting contrast enhancement in cross-sectional OCT images using methylene blue dye are presented. The second method is an optical switch suppression technique based on the use of a transmembrane protein called bacteriorhodopsin. Initial experiments indicate that biochemical optical switches, such as bacteriorhodopsin, are excellent contrast agent candidates for molecular contrast OCT.

Full Text

Duke Authors

Cited Authors

  • Yang, C; Rao, KD; Choma, M; Yazdanfar, S; Rollins, AM; Izatt, JA

Published Date

  • January 1, 2003

Published In

Volume / Issue

  • 5140 /

Start / End Page

  • 95 - 100

International Standard Serial Number (ISSN)

  • 0277-786X

Digital Object Identifier (DOI)

  • 10.1117/12.501007

Citation Source

  • Scopus