Noninvasive quantitation of blood flow in the retinal microcirculation may elucidate the progression and treatment of ocular disorders including diabetic retinopathy, age-related macular degeneration, and glaucoma. Color Doppler optical coherence tomography (CDOCT) was recently introduced as a technique allowing simultaneous micron-scale resolution cross-sectional imaging of tissue microstructure and blood flow in the human retina. Here, time-resolved imaging of dynamics of blood flow profiles was performed to measure cardiac pulsatility within retinal vessels. Retinal pulsatility has been shown to decrease throughout the progression of diabetic retinopathy.