Phase-referenced interferometer with subwavelength and subhertz sensitivity applied to the study of cell membrane dynamics


Journal Article

We report a highly sensitive means of measuring cellular dynamics with a novel interferometer that can measure motional phase changes. The system is based on a modified Michelson interferometer with a composite laser beam of 1550-nm low-coherence light and 775-nm CW light. The sample is prepared on a coverslip that is highly reflective at 775 nm. By referencing the heterodyne phase of the 1550-nm light reflected from the sample to that of the 775-nm light reflected from the coverslip, small motions in the sample are detected, and motional artifacts from vibrations in the interferometer are completely eliminated. We demonstrate that the system is sensitive to motions as small as 3.6 nm and velocities as small as 1 nm/s. Using the instrument, we study transient volume changes of a few (approximately three) cells in a monolayer immersed in weakly hypotonie and hypertonic solutions. © 2001 Optical Society of America.

Full Text

Duke Authors

Cited Authors

  • Yang, C; Wax, A; Hahn, MS; Badizadegan, K; Dasari, RR; Feld, MS

Published Date

  • August 15, 2001

Published In

Volume / Issue

  • 26 / 16

Start / End Page

  • 1271 - 1273

International Standard Serial Number (ISSN)

  • 0146-9592

Digital Object Identifier (DOI)

  • 10.1364/OL.26.001271

Citation Source

  • Scopus