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Phase-referenced interferometer with subwavelength and subhertz sensitivity applied to the study of cell membrane dynamics

Publication ,  Journal Article
Yang, C; Wax, A; Hahn, MS; Badizadegan, K; Dasari, RR; Feld, MS
Published in: Optics Letters
August 15, 2001

We report a highly sensitive means of measuring cellular dynamics with a novel interferometer that can measure motional phase changes. The system is based on a modified Michelson interferometer with a composite laser beam of 1550-nm low-coherence light and 775-nm CW light. The sample is prepared on a coverslip that is highly reflective at 775 nm. By referencing the heterodyne phase of the 1550-nm light reflected from the sample to that of the 775-nm light reflected from the coverslip, small motions in the sample are detected, and motional artifacts from vibrations in the interferometer are completely eliminated. We demonstrate that the system is sensitive to motions as small as 3.6 nm and velocities as small as 1 nm/s. Using the instrument, we study transient volume changes of a few (approximately three) cells in a monolayer immersed in weakly hypotonie and hypertonic solutions. © 2001 Optical Society of America.

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Published In

Optics Letters

DOI

ISSN

0146-9592

Publication Date

August 15, 2001

Volume

26

Issue

16

Start / End Page

1271 / 1273

Related Subject Headings

  • Optics
  • 0906 Electrical and Electronic Engineering
  • 0206 Quantum Physics
  • 0205 Optical Physics
 

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Yang, C., Wax, A., Hahn, M. S., Badizadegan, K., Dasari, R. R., & Feld, M. S. (2001). Phase-referenced interferometer with subwavelength and subhertz sensitivity applied to the study of cell membrane dynamics. Optics Letters, 26(16), 1271–1273. https://doi.org/10.1364/OL.26.001271
Yang, C., A. Wax, M. S. Hahn, K. Badizadegan, R. R. Dasari, and M. S. Feld. “Phase-referenced interferometer with subwavelength and subhertz sensitivity applied to the study of cell membrane dynamics.” Optics Letters 26, no. 16 (August 15, 2001): 1271–73. https://doi.org/10.1364/OL.26.001271.
Yang C, Wax A, Hahn MS, Badizadegan K, Dasari RR, Feld MS. Phase-referenced interferometer with subwavelength and subhertz sensitivity applied to the study of cell membrane dynamics. Optics Letters. 2001 Aug 15;26(16):1271–3.
Yang, C., et al. “Phase-referenced interferometer with subwavelength and subhertz sensitivity applied to the study of cell membrane dynamics.” Optics Letters, vol. 26, no. 16, Aug. 2001, pp. 1271–73. Scopus, doi:10.1364/OL.26.001271.
Yang C, Wax A, Hahn MS, Badizadegan K, Dasari RR, Feld MS. Phase-referenced interferometer with subwavelength and subhertz sensitivity applied to the study of cell membrane dynamics. Optics Letters. 2001 Aug 15;26(16):1271–1273.
Journal cover image

Published In

Optics Letters

DOI

ISSN

0146-9592

Publication Date

August 15, 2001

Volume

26

Issue

16

Start / End Page

1271 / 1273

Related Subject Headings

  • Optics
  • 0906 Electrical and Electronic Engineering
  • 0206 Quantum Physics
  • 0205 Optical Physics