Nuclear morphology measurements using Fourier domain low coherence interferometry.


Journal Article

We present a new common path configuration Fourier domain low coherence interferometry (fLCI) optical system and demonstrate its capabilities by presenting results which determine the size of cell nuclei in a monolayer of T84 epithelial cells. The optical system uses a white light source in a modified Michelson interferometer and a spectrograph for detection of the mixed signal and reference fields. Depth resolution is obtained from the Fourier transform of the measured spectrum which provides the axial spatial cross-correlation between the signal and reference fields. The spectral dependence of scattering by the samples is determined by windowing the spectrum to measure the scattering amplitude as a function of wavenumber. We present evidence that fLCI accurately measures the longitudinal profile of cell nuclei rather than the transverse profile.

Full Text

Duke Authors

Cited Authors

  • Graf, RN; Wax, A

Published Date

  • June 2005

Published In

Volume / Issue

  • 13 / 12

Start / End Page

  • 4693 - 4698

PubMed ID

  • 16429608

Pubmed Central ID

  • 16429608

Electronic International Standard Serial Number (EISSN)

  • 1094-4087

International Standard Serial Number (ISSN)

  • 1094-4087

Digital Object Identifier (DOI)

  • 10.1364/opex.13.004693


  • eng