Feasibility of field-based light scattering spectroscopy.
Journal Article
Light scattering spectroscopy (LSS) is a new technique capable of accurately measuring the features of nuclei and other cellular organelles in situ. We present the considerations required to implement and interpret field-based detection in LSS, where the scattered electric field is detected interferometrically, and demonstrate that the technique is experimentally feasible. A theoretical formalism for modeling field-based LSS signals based on Mie scattering is presented. Phase-front uniformity is shown to play an important and novel role. Results of heterodyne experiments with polystyrene microspheres that localize LSS signals to a region about 30 microns in axial extent are reported. In addition, differences between field-based LSS and the earlier intensity-based LSS are discussed.
Full Text
Duke Authors
Cited Authors
- Yang, C; Perelman, LT; Wax, A; Dasari, RR; Feld, MS
Published Date
- April 2000
Published In
Volume / Issue
- 5 / 2
Start / End Page
- 138 - 143
PubMed ID
- 10938777
Pubmed Central ID
- 10938777
Electronic International Standard Serial Number (EISSN)
- 1560-2281
International Standard Serial Number (ISSN)
- 1083-3668
Digital Object Identifier (DOI)
- 10.1117/1.429980
Language
- eng