Feasibility of field-based light scattering spectroscopy.

Published

Journal Article

Light scattering spectroscopy (LSS) is a new technique capable of accurately measuring the features of nuclei and other cellular organelles in situ. We present the considerations required to implement and interpret field-based detection in LSS, where the scattered electric field is detected interferometrically, and demonstrate that the technique is experimentally feasible. A theoretical formalism for modeling field-based LSS signals based on Mie scattering is presented. Phase-front uniformity is shown to play an important and novel role. Results of heterodyne experiments with polystyrene microspheres that localize LSS signals to a region about 30 microns in axial extent are reported. In addition, differences between field-based LSS and the earlier intensity-based LSS are discussed.

Full Text

Duke Authors

Cited Authors

  • Yang, C; Perelman, LT; Wax, A; Dasari, RR; Feld, MS

Published Date

  • April 2000

Published In

Volume / Issue

  • 5 / 2

Start / End Page

  • 138 - 143

PubMed ID

  • 10938777

Pubmed Central ID

  • 10938777

Electronic International Standard Serial Number (EISSN)

  • 1560-2281

International Standard Serial Number (ISSN)

  • 1083-3668

Digital Object Identifier (DOI)

  • 10.1117/1.429980

Language

  • eng