Low-cost, scalable laser scanning module for real-time reflectance and fluorescence confocal microscopy.

Journal Article (Journal Article)

We present a low-cost, high-speed, retrofitted laser scanning module for microscopy. The cage-mounted system, with various available fiber-coupled sources, offers a real-time imaging alternative to costly commercial systems with capabilities for conventional or confocal reflectance and fluorescence applications as well as advanced laser scanning microscopy implementations. Reflectance images of a resolution target and confocal images of fluorescent polystyrene beads are presented for system characterization. Confocal fluorescence image stacks of T84 epithelial cancer cells are presented to demonstrate application to biological studies. This laser scanning module is a flexible, scalable, high-speed alternative to commercial laser scanning systems suitable for applications requiring a simple imaging tool and for teaching laboratories.

Full Text

Duke Authors

Cited Authors

  • Chou, DR; Bower, BA; Wax, A

Published Date

  • April 2005

Published In

Volume / Issue

  • 44 / 11

Start / End Page

  • 2013 - 2018

PubMed ID

  • 15835349

Electronic International Standard Serial Number (EISSN)

  • 1539-4522

International Standard Serial Number (ISSN)

  • 1559-128X

Digital Object Identifier (DOI)

  • 10.1364/ao.44.002013


  • eng