Cellular organization and substructure measured using angle-resolved low-coherence interferometry.

Journal Article (Journal Article)

We measure the organization and substructure of HT29 epithelial cells in a monolayer using angle-resolved low-coherence interferometry. This new technique probes cellular structure by measuring scattered light, as in flow cytometry, but offers an advantage in that the structure can be examined in situ, avoiding the need to disrupt the cell monolayer. We determine the size distribution of the cell nuclei by fitting measured light-scattering spectra to the predictions of Mie theory. In addition, we obtain information about the cellular organization and substructure by examining the spatial correlations within the monolayer. A remarkable finding is that the spatial correlations over small length scales take the form of an inverse power law, indicating the fractal nature of the packing of the subcellular structures. We also identify spatial correlations on a scale large compared with the size of a cell, indicating an overlying order within the monolayer.

Full Text

Duke Authors

Cited Authors

  • Wax, A; Yang, C; Backman, V; Badizadegan, K; Boone, CW; Dasari, RR; Feld, MS

Published Date

  • April 2002

Published In

Volume / Issue

  • 82 / 4

Start / End Page

  • 2256 - 2264

PubMed ID

  • 11916880

Pubmed Central ID

  • PMC1302018

Electronic International Standard Serial Number (EISSN)

  • 1542-0086

International Standard Serial Number (ISSN)

  • 0006-3495

Digital Object Identifier (DOI)

  • 10.1016/s0006-3495(02)75571-9


  • eng