FRET based biosensor for detection of active NF-kB
Published
Journal Article
The Nuclear Factor kB is a transcription factor, ubiquitously expressed, involved in the regulation of a large number of genes and in a variety of human disease including inflammation, asthma, atherosclerosis, AIDS, septic shock, arthritis and cancer. The critical need for a simple and direct method to evaluate the quantity of active NF-kB in a biological sample can be addressed using a suitable and reusable biosensor. For this purpose, a novel method, using fluorescence resonance energy transfer (FRET), to detect the active form of NF-kB binding a specific DNA sequence has been developed. A single-stranded DNA (ssDNA) with auto-complementary sequence has been properly designed and synthesized. In order to evaluate FRET due to the DNA/protein binding interaction taking place between double-stranded DNA (dsDNA) immobilized in a capillary wall and NF-kB proteins, a highly sensitive FRET-based biosensor system developed in our laboratory was used. Preliminary results show that our system was capable of detecting the active form of NF-kB protein with a detection efficiency of about 90% and that the system has a good regenerability.
Full Text
Duke Authors
Cited Authors
- Baldini, F; Citti, L; Domenici, C; Giannetti, A; Tedeschi, L; Vo-Dinh, T; Wabuyele, MB
Published Date
- December 13, 2005
Published In
Volume / Issue
- 5855 PART I /
Start / End Page
- 439 - 442
International Standard Serial Number (ISSN)
- 0277-786X
Digital Object Identifier (DOI)
- 10.1117/12.623696
Citation Source
- Scopus