A compact CMOS biochip immunosensor towards the detection of a single bacteria.

Journal Article (Journal Article)

Recent use of biological warfare (BW) agents has led to a growing interest in the rapid and sensitive detection of pathogens. Therefore, the development of field-usable detection devices for sensitive and selective detection of BW agents is an important issue. In this work, we report a portable biochip system based on complementary metal oxide semiconductor (CMOS) technology that has great potential as a device for single-bacteria detection. The possibility of single-bacteria detection is reported using an immunoassay coupled to laser-induced fluorescence (LIF) detection. Bacillus globigii spores, which are a surrogate species for B. anthracis spores, were used as the test sample. Enzymatic amplification following immunocomplex formation allowed remarkably sensitive detection of B. globigii spores, and could preclude a complicated optical and instrumental system usually required for high-sensitive detection. Atomic force microscopy (AFM) was employed to investigate whether B. globigii spores detected in the portable biochip system exist in single-cell or multicellular form. It was found that B. globigii spores mostly exist in multicellular form with a small minority of single-cell form. The results showed that the portable biochip system has great potential as a device for single-particle or possibly even single-organism detection.

Full Text

Duke Authors

Cited Authors

  • Song, JM; Culha, M; Kasili, PM; Griffin, GD; Vo-Dinh, T

Published Date

  • May 2005

Published In

Volume / Issue

  • 20 / 11

Start / End Page

  • 2203 - 2209

PubMed ID

  • 15797317

Electronic International Standard Serial Number (EISSN)

  • 1873-4235

International Standard Serial Number (ISSN)

  • 0956-5663

Digital Object Identifier (DOI)

  • 10.1016/j.bios.2004.08.033


  • eng