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Principle of synchronous luminescence (SL) technique for biomedical diagnostics

Publication ,  Journal Article
Vo-Dinh, T
Published in: Proceedings of SPIE - The International Society for Optical Engineering
January 1, 2000

In the present study, the principle of synchronous luminescence (SL) is described for use in biomedical diagnostics. The SL method involves scanning simultaneously both emission and excitation wavelengths while keeping a constant wavelength interval between them. This SF procedure simplifies the emission spectrum and provides for greater selectivity and is used to detect subtle differences in the fluorescence emission of the biochemical species of cells and tissues. The SL method can be use to analyze tissue in vivo or to investigate spectral differences in normal and neoplastic cells in vitro. SL scans of skin tissues illustrate the use of the method. For in vitro diagnosis, a difference between the fluorescent spectra of the normal rat liver epithelial (RLE) and hepatoma cell lines were detected using synchronous fluorescence. The potential use of SF as a screening tool for cancer diagnosis is discussed.

Duke Scholars

Published In

Proceedings of SPIE - The International Society for Optical Engineering

ISSN

0277-786X

Publication Date

January 1, 2000

Volume

3911

Start / End Page

42 / 49

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4009 Electronics, sensors and digital hardware
  • 4006 Communications engineering
 

Citation

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Vo-Dinh, T. (2000). Principle of synchronous luminescence (SL) technique for biomedical diagnostics. Proceedings of SPIE - The International Society for Optical Engineering, 3911, 42–49.
Vo-Dinh, T. “Principle of synchronous luminescence (SL) technique for biomedical diagnostics.” Proceedings of SPIE - The International Society for Optical Engineering 3911 (January 1, 2000): 42–49.
Vo-Dinh T. Principle of synchronous luminescence (SL) technique for biomedical diagnostics. Proceedings of SPIE - The International Society for Optical Engineering. 2000 Jan 1;3911:42–9.
Vo-Dinh, T. “Principle of synchronous luminescence (SL) technique for biomedical diagnostics.” Proceedings of SPIE - The International Society for Optical Engineering, vol. 3911, Jan. 2000, pp. 42–49.
Vo-Dinh T. Principle of synchronous luminescence (SL) technique for biomedical diagnostics. Proceedings of SPIE - The International Society for Optical Engineering. 2000 Jan 1;3911:42–49.

Published In

Proceedings of SPIE - The International Society for Optical Engineering

ISSN

0277-786X

Publication Date

January 1, 2000

Volume

3911

Start / End Page

42 / 49

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4009 Electronics, sensors and digital hardware
  • 4006 Communications engineering