Application of an integrated microchip system with capillary array electrophoresis to optimization of enzymatic reactions


Journal Article

In this work, a combination of complementary metal-oxide semiconductor (CMOS) microchip system with capillary array electrophoresis (CAE) is demonstrated as a system for optimizing conditions for enzymatic reaction. Dimethylacridinone (DDAO)-phosphate substrate and alkaline phosphatase conjugate were selected for the enzymatic reaction, which was applicable to the enzyme-linked immunosorbent assay (ELISA) technique. Laser-induced fluorometry with a miniature semiconductor laser was used to detect the enzymatic products. The speed of the enzymatic reaction between the DDAO-phosphate and the alkaline phosphatase conjugate was investigated as a function of reaction time. The microchip-CAE detection system could determine the pH condition and the concentration of enzyme that are suitable for rapid and low-cost analysis. This result shows the feasibility of using the microchip-CAE system for application to miniaturized screening systems. © 2003 Elsevier Science B.V. All rights reserved.

Full Text

Duke Authors

Cited Authors

  • Song, JM; Griffin, GD; Vo-Dinh, T

Published Date

  • July 1, 2003

Published In

Volume / Issue

  • 487 / 1

Start / End Page

  • 75 - 82

International Standard Serial Number (ISSN)

  • 0003-2670

Digital Object Identifier (DOI)

  • 10.1016/S0003-2670(03)00515-4

Citation Source

  • Scopus