Use of transmitted room-temperature phosphorescence to improve nalidixic acid determination

Journal Article (Journal Article)

A method for nalidixic acid (NA) determination by transmitted room-temperature phosphorescence is proposed. The antibacterial agent NA in acidic solution shows native fluorescence; when NA is spotted on a filter paper precoated with β-cyclodextrin and lead (II) acetate and dried for 5 min, phosphorescence results. The transmitted phosphorescence was measured directly with the use of a homemade sample holder. The excitation and emission wavelengths were 330 and 417 nm, respectively. The applicable concentration range was between 0.06 and 4.50 μg/mL with a relative standard deviation of 2.8% and a detection limit of 0.02 μg/mL. The method was applied to the determination of NA in human urine and pharmaceuticals, with recoveries from 97.5 to 105.5%.

Full Text

Duke Authors

Cited Authors

  • Capitán-Vallvey, LF; Ojeda, F; Del Olmo, M; Avidad, R; Navalón, A; Vo-Dinh, T

Published Date

  • January 1, 1998

Published In

Volume / Issue

  • 52 / 1

Start / End Page

  • 101 - 105

International Standard Serial Number (ISSN)

  • 0003-7028

Digital Object Identifier (DOI)

  • 10.1366/0003702981942393

Citation Source

  • Scopus