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Screening for the breast cancer gene (BRCA1) using a biochip system and molecular beacon probes immobilized on solid surfaces.

Publication ,  Journal Article
Culha, M; Stokes, DL; Griffin, GD; Vo-Dinh, T
Published in: Journal of biomedical optics
May 2004

We describe the use of a biochip based on complementary metal oxide semiconductor (CMOS) technology for detection of specific genetic sequences using molecular beacons (MB) immobilized on solid surfaces as probes. The applicability of this miniature detection system for screening for the BRCA1 gene is evaluated using MB probes, designed especially for the BRCA1 gene. MB probes are immobilized on a zeta-probe membrane by biotin-streptavidin immobilization. Two immobilization strategies are investigated to obtain optimal assay sensitivity. The MB is immobilized by manual spotting on zeta-probe membrane surfaces with the use of a custom-made stamping system. The detection of the BRCA1 gene using an MB probe is successfully demonstrated and expands the use of the CMOS biochip for medical applications.

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Published In

Journal of biomedical optics

DOI

EISSN

1560-2281

ISSN

1083-3668

Publication Date

May 2004

Volume

9

Issue

3

Start / End Page

439 / 443

Related Subject Headings

  • Spectrometry, Fluorescence
  • Sensitivity and Specificity
  • Reproducibility of Results
  • Optics
  • Oligonucleotide Probes
  • Oligonucleotide Array Sequence Analysis
  • Membranes, Artificial
  • Humans
  • Genetic Testing
  • Genetic Predisposition to Disease
 

Citation

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Culha, M., Stokes, D. L., Griffin, G. D., & Vo-Dinh, T. (2004). Screening for the breast cancer gene (BRCA1) using a biochip system and molecular beacon probes immobilized on solid surfaces. Journal of Biomedical Optics, 9(3), 439–443. https://doi.org/10.1117/1.1691025
Culha, Mustafa, David L. Stokes, Guy D. Griffin, and Tuan Vo-Dinh. “Screening for the breast cancer gene (BRCA1) using a biochip system and molecular beacon probes immobilized on solid surfaces.Journal of Biomedical Optics 9, no. 3 (May 2004): 439–43. https://doi.org/10.1117/1.1691025.
Culha M, Stokes DL, Griffin GD, Vo-Dinh T. Screening for the breast cancer gene (BRCA1) using a biochip system and molecular beacon probes immobilized on solid surfaces. Journal of biomedical optics. 2004 May;9(3):439–43.
Culha, Mustafa, et al. “Screening for the breast cancer gene (BRCA1) using a biochip system and molecular beacon probes immobilized on solid surfaces.Journal of Biomedical Optics, vol. 9, no. 3, May 2004, pp. 439–43. Epmc, doi:10.1117/1.1691025.
Culha M, Stokes DL, Griffin GD, Vo-Dinh T. Screening for the breast cancer gene (BRCA1) using a biochip system and molecular beacon probes immobilized on solid surfaces. Journal of biomedical optics. 2004 May;9(3):439–443.

Published In

Journal of biomedical optics

DOI

EISSN

1560-2281

ISSN

1083-3668

Publication Date

May 2004

Volume

9

Issue

3

Start / End Page

439 / 443

Related Subject Headings

  • Spectrometry, Fluorescence
  • Sensitivity and Specificity
  • Reproducibility of Results
  • Optics
  • Oligonucleotide Probes
  • Oligonucleotide Array Sequence Analysis
  • Membranes, Artificial
  • Humans
  • Genetic Testing
  • Genetic Predisposition to Disease