Charge-coupled device fluorescence detection for capillary-zone electrophoresis (CCD-CZE)

Journal Article (Journal Article)

This paper describes a new instrument system combining capillary-zone electrophoresis (CZE) separation and charge-coupled device (CCD) fluorescence detection. The performance of this CCD-CZE system is evaluated and compared with the performance of a photomultiplier tube (PMT) detector, with the use of signal-to-noise (S/N) ratio and acquisition time as measures of performance. The feasibility of on-column fluorescence spectrometric detection is demonstrated, and a three-dimensional electrophorogram is presented to show the spectral and time resolution obtained by injection of 2 femtomoles (5 nanoliters of 4.0 × 10-7 M) of fluorescein into the capillary column. The software us a 'peak sum' method to achieve optimum S/N ration.

Full Text

Duke Authors

Cited Authors

  • Cheng, YF; Piccard, RD; Vo-Dinh, T

Published Date

  • January 1, 1990

Published In

Volume / Issue

  • 44 / 5

Start / End Page

  • 755 - 765

International Standard Serial Number (ISSN)

  • 0003-7028

Digital Object Identifier (DOI)

  • 10.1366/0003702904087082

Citation Source

  • Scopus