Skip to main content

Nanosensor for in vivo measurement of the carcinogen benzo[a]pyrene in a single cell.

Publication ,  Journal Article
Kasili, PM; Cullum, BM; Griffin, GD; Vo-Dinh, T
Published in: Journal of nanoscience and nanotechnology
December 2002

This work describes the fabrication and the application of an antibody-based fiber-optic nanosensor for in situ measurements of the carcinogen benzo[a]pyrene (BaP) in a single cell. This antibody-based spectroscopic nanosensor is miniaturized enabling the detection of fluorescent analytes in single cells. In addition to measuring fluorescent analytes in single cells, the nanosensor has the potential to be applied for both diagnostic and proteomics purposes. In this work, the human breast carcinoma cell line, MCF-7, was used as the model system to perform BaP measurements in single cells. A standard concentration curve for BaP was established and used to perform quantitative analyses of BaP in individual cells. From these analyses, it was estimated that the concentration of BaP in the individual cells investigated was approximately 3.61 x 10(-10) M. The results obtained demonstrate the application of antibody-based nanosensors for performing in situ measurements inside a single cell.

Duke Scholars

Published In

Journal of nanoscience and nanotechnology

DOI

EISSN

1533-4899

ISSN

1533-4880

Publication Date

December 2002

Volume

2

Issue

6

Start / End Page

653 / 658

Related Subject Headings

  • Tumor Cells, Cultured
  • Sensitivity and Specificity
  • Reproducibility of Results
  • Optical Fibers
  • Nanotechnology
  • Nanoscience & Nanotechnology
  • Microscopy, Fluorescence
  • Humans
  • Fiber Optic Technology
  • Equipment Design
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kasili, P. M., Cullum, B. M., Griffin, G. D., & Vo-Dinh, T. (2002). Nanosensor for in vivo measurement of the carcinogen benzo[a]pyrene in a single cell. Journal of Nanoscience and Nanotechnology, 2(6), 653–658. https://doi.org/10.1166/153348802321105941
Kasili, P. M., B. M. Cullum, G. D. Griffin, and T. Vo-Dinh. “Nanosensor for in vivo measurement of the carcinogen benzo[a]pyrene in a single cell.Journal of Nanoscience and Nanotechnology 2, no. 6 (December 2002): 653–58. https://doi.org/10.1166/153348802321105941.
Kasili PM, Cullum BM, Griffin GD, Vo-Dinh T. Nanosensor for in vivo measurement of the carcinogen benzo[a]pyrene in a single cell. Journal of nanoscience and nanotechnology. 2002 Dec;2(6):653–8.
Kasili, P. M., et al. “Nanosensor for in vivo measurement of the carcinogen benzo[a]pyrene in a single cell.Journal of Nanoscience and Nanotechnology, vol. 2, no. 6, Dec. 2002, pp. 653–58. Epmc, doi:10.1166/153348802321105941.
Kasili PM, Cullum BM, Griffin GD, Vo-Dinh T. Nanosensor for in vivo measurement of the carcinogen benzo[a]pyrene in a single cell. Journal of nanoscience and nanotechnology. 2002 Dec;2(6):653–658.

Published In

Journal of nanoscience and nanotechnology

DOI

EISSN

1533-4899

ISSN

1533-4880

Publication Date

December 2002

Volume

2

Issue

6

Start / End Page

653 / 658

Related Subject Headings

  • Tumor Cells, Cultured
  • Sensitivity and Specificity
  • Reproducibility of Results
  • Optical Fibers
  • Nanotechnology
  • Nanoscience & Nanotechnology
  • Microscopy, Fluorescence
  • Humans
  • Fiber Optic Technology
  • Equipment Design