Comparison of Multichannel SIT Image Vidicon and Photomultiplier Sequential Linear Scanning Systems for the Measurement of Steady-State and Transient Fluorescence of Molecules in Solution


Journal Article

The feasibility of using the SIT Image vidicon as a potential detector for liquid chromatography Is demonstrated. Limits of detection range from ~2 × 102 ng for anthracene to ~6 × 10-1 ng for riboflavin. A direct experimental comparison Is made of a silicon Intensified target vidicon multichannel (SIT-MC) and a photomultiplier sequential linear scanning (PM-SLS) system for the detection of steady-state fluorescence from molecules In solution. A discussion is given of the fundamental differences between the ways the two systems acquire, process, and read out data. It Is concluded that the PM system is slightly better than the SIT for measuring steady-state fluorescence; on the other hand, the multichannel advantage of the SIT would appear to make it the better system for measuring transient fluorescence signals. © 1977, American Chemical Society. All rights reserved.

Full Text

Duke Authors

Cited Authors

  • Cooney, RP; Vo-Dinh, TD; Walden, G; Winefordner, JD

Published Date

  • June 1, 1977

Published In

Volume / Issue

  • 49 / 7

Start / End Page

  • 939 - 943

Electronic International Standard Serial Number (EISSN)

  • 1520-6882

International Standard Serial Number (ISSN)

  • 0003-2700

Digital Object Identifier (DOI)

  • 10.1021/ac50015a017

Citation Source

  • Scopus