Surface-enhanced Raman scattering substrate based on a self-assembled monolayer for use in gene diagnostics.
Published
Journal Article
The development of surface-enhanced Raman scattering (SERS)-active substrates for cancer gene detection is described. The detection method uses Raman active dye-labeled DNA gene probes, self-assembled monolayers, and nanostructured metallic substrates as SERS-active platforms. The mercaptohexane-labeled single-stranded DNA (SH-(CH(2))(6)-ssDNA)/6-mercapto-1-hexanol system formed on a silver surface is characterized by atomic force microscopy. The surface-enhanced Raman gene (SERGen) probes developed in this study can be used to detect DNA targets via hybridization to complementary DNA probes. The probes do not require the use of radioactive labels and have a great potential to provide both sensitivity and selectivity. The effectiveness of this approach and its application in cancer gene diagnostics (BRCA1 breast cancer gene) are investigated.
Full Text
Duke Authors
Cited Authors
- Culha, M; Stokes, D; Allain, LR; Vo-Dinh, T
Published Date
- November 2003
Published In
Volume / Issue
- 75 / 22
Start / End Page
- 6196 - 6201
PubMed ID
- 14616001
Pubmed Central ID
- 14616001
Electronic International Standard Serial Number (EISSN)
- 1520-6882
International Standard Serial Number (ISSN)
- 0003-2700
Digital Object Identifier (DOI)
- 10.1021/ac0346003
Language
- eng