Surface-enhanced Raman scattering substrate based on a self-assembled monolayer for use in gene diagnostics.

Journal Article (Journal Article)

The development of surface-enhanced Raman scattering (SERS)-active substrates for cancer gene detection is described. The detection method uses Raman active dye-labeled DNA gene probes, self-assembled monolayers, and nanostructured metallic substrates as SERS-active platforms. The mercaptohexane-labeled single-stranded DNA (SH-(CH(2))(6)-ssDNA)/6-mercapto-1-hexanol system formed on a silver surface is characterized by atomic force microscopy. The surface-enhanced Raman gene (SERGen) probes developed in this study can be used to detect DNA targets via hybridization to complementary DNA probes. The probes do not require the use of radioactive labels and have a great potential to provide both sensitivity and selectivity. The effectiveness of this approach and its application in cancer gene diagnostics (BRCA1 breast cancer gene) are investigated.

Full Text

Duke Authors

Cited Authors

  • Culha, M; Stokes, D; Allain, LR; Vo-Dinh, T

Published Date

  • November 2003

Published In

Volume / Issue

  • 75 / 22

Start / End Page

  • 6196 - 6201

PubMed ID

  • 14616001

Electronic International Standard Serial Number (EISSN)

  • 1520-6882

International Standard Serial Number (ISSN)

  • 0003-2700

Digital Object Identifier (DOI)

  • 10.1021/ac0346003


  • eng