Miniature biochip system for bioenvironmental applications


Journal Article

An integrated multi-functional biochip based on integrated circuit complementary metal oxide semiconductor (CMOS) sensor array for use in medical diagnostics and pathogen detection has been described. The usefulness and potential of the biochip as a rapid, inexpensive screening tool for detection of bioenvironmental pathogens will be demonstrated. Detection of aerosolized spores was achieved by coupling the miniature system to a portable bioaerosol sampler, and the performance of the antibody-based recognition and enzyme amplification method was evaluated. The bioassay performance was found to be compatible with the air sampling device, and the enzymatic amplification was found to be an attractive amplification method for detection of low spore concentrations. The combined portable bioaerosol sampler and miniature biochip system detected 100 B. globigii spores, corresponding to 17 aerosolized spores/L of air.

Full Text

Duke Authors

Cited Authors

  • Wabuyele, MB; Yan, F; Martin, ME; Griffin, GD; Stokes, DL; Stratis-Cullum, DN; Mobley, J; Vass, AA; Vo-Dinh, T

Published Date

  • December 1, 2004

Published In

Volume / Issue

  • 5586 /

Start / End Page

  • 26 - 32

International Standard Serial Number (ISSN)

  • 0277-786X

Digital Object Identifier (DOI)

  • 10.1117/12.580126

Citation Source

  • Scopus