Multi-analyte analysis system using an antibody-based biochip.

Journal Article (Journal Article)

A multi-analyte detection system using a unique antibody (Ab) biochip is described. The Ab-based biochip, also referred to as the protein biochip, uses a sensor array based on a complementary metal oxide silicon (CMOS) integrated circuit. The Ab-biochip has a sampling platform of four-by-four microarrays of antibodies deposited onto a Nylon membrane substrate. The micro-arrayed antibodies can be interrogated simultaneously or sequentially using the biochip sensing array detector with the use of a diffractive optical element illuminating each antibody spot individually. The usefulness of the Ab biochip is illustrated by the measurements of immunoglobulin G (IgG) used as the model analyte system. The detection limit for Cy5-labeled IgG molecules was 13 pg.

Full Text

Duke Authors

Cited Authors

  • Moreno-Bondi, MC; Alarie, JP; Vo-Dinh, T

Published Date

  • January 2003

Published In

Volume / Issue

  • 375 / 1

Start / End Page

  • 120 - 124

PubMed ID

  • 12520447

Electronic International Standard Serial Number (EISSN)

  • 1618-2650

International Standard Serial Number (ISSN)

  • 1618-2642

Digital Object Identifier (DOI)

  • 10.1007/s00216-002-1626-y


  • eng