Multi-analyte analysis system using an antibody-based biochip.
Journal Article (Journal Article)
A multi-analyte detection system using a unique antibody (Ab) biochip is described. The Ab-based biochip, also referred to as the protein biochip, uses a sensor array based on a complementary metal oxide silicon (CMOS) integrated circuit. The Ab-biochip has a sampling platform of four-by-four microarrays of antibodies deposited onto a Nylon membrane substrate. The micro-arrayed antibodies can be interrogated simultaneously or sequentially using the biochip sensing array detector with the use of a diffractive optical element illuminating each antibody spot individually. The usefulness of the Ab biochip is illustrated by the measurements of immunoglobulin G (IgG) used as the model analyte system. The detection limit for Cy5-labeled IgG molecules was 13 pg.
Full Text
Duke Authors
Cited Authors
- Moreno-Bondi, MC; Alarie, JP; Vo-Dinh, T
Published Date
- January 2003
Published In
Volume / Issue
- 375 / 1
Start / End Page
- 120 - 124
PubMed ID
- 12520447
Electronic International Standard Serial Number (EISSN)
- 1618-2650
International Standard Serial Number (ISSN)
- 1618-2642
Digital Object Identifier (DOI)
- 10.1007/s00216-002-1626-y
Language
- eng