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PORTABLE FIBEROPTIC MONITOR FOR FLUORIMETRIC BIOASSAYS.

Publication ,  Journal Article
Vo-Dinh, T; Griffin, GD; Ambrose, KR
Published in: Applied Spectroscopy
January 1, 1986
Published version (DOI)

A simple and portable fiberoptic instrument for fluorimetric bioassays is described. The instrument is designed to be readily adaptable to commercially available biotesting wells on microplates. The microwells can also be used as interchangeable sensor heads. The utility of the fiber-optic biosensor is illustrated with a model enzyme-linked immunosorbent assay (ELISA) used to measure varying amounts of rabbit immunoglobulin G from 10 pg to 1 mu g. The detection method utilizes an enzyme-amplified immunoassay of 4-methylumbelliferone phosphate as the enzyme substrate. The sensitivity of this assay is three orders of magnitude better than that obtained with a conventional ELISA absorption spectrometric assay using a commercially available reader.

Duke Scholars

Tuan Vo-Dinh
Author Tuan Vo-Dinh Biomedical Engineering
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Published In

Applied Spectroscopy

DOI

10.1366/0003702864508575

ISSN

0003-7028

Publication Date

January 1, 1986

Volume

40

Issue

5

Start / End Page

696 / 700

Related Subject Headings

  • Analytical Chemistry
  • 0913 Mechanical Engineering
  • 0306 Physical Chemistry (incl. Structural)
  • 0301 Analytical Chemistry
 

Citation

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Vo-Dinh, T., Griffin, G. D., & Ambrose, K. R. (1986). PORTABLE FIBEROPTIC MONITOR FOR FLUORIMETRIC BIOASSAYS. Applied Spectroscopy, 40(5), 696–700. https://doi.org/10.1366/0003702864508575
Vo-Dinh, T., G. D. Griffin, and K. R. Ambrose. “PORTABLE FIBEROPTIC MONITOR FOR FLUORIMETRIC BIOASSAYS.” Applied Spectroscopy 40, no. 5 (January 1, 1986): 696–700. https://doi.org/10.1366/0003702864508575.
Vo-Dinh T, Griffin GD, Ambrose KR. PORTABLE FIBEROPTIC MONITOR FOR FLUORIMETRIC BIOASSAYS. Applied Spectroscopy. 1986 Jan 1;40(5):696–700.
Vo-Dinh, T., et al. “PORTABLE FIBEROPTIC MONITOR FOR FLUORIMETRIC BIOASSAYS.” Applied Spectroscopy, vol. 40, no. 5, Jan. 1986, pp. 696–700. Scopus, doi:10.1366/0003702864508575.
Vo-Dinh T, Griffin GD, Ambrose KR. PORTABLE FIBEROPTIC MONITOR FOR FLUORIMETRIC BIOASSAYS. Applied Spectroscopy. 1986 Jan 1;40(5):696–700.
Journal cover image

Published In

Applied Spectroscopy

DOI

10.1366/0003702864508575

ISSN

0003-7028

Publication Date

January 1, 1986

Volume

40

Issue

5

Start / End Page

696 / 700

Related Subject Headings

  • Analytical Chemistry
  • 0913 Mechanical Engineering
  • 0306 Physical Chemistry (incl. Structural)
  • 0301 Analytical Chemistry