Journal Article

A simple and portable fiberoptic instrument for fluorimetric bioassays is described. The instrument is designed to be readily adaptable to commercially available biotesting wells on microplates. The microwells can also be used as interchangeable sensor heads. The utility of the fiber-optic biosensor is illustrated with a model enzyme-linked immunosorbent assay (ELISA) used to measure varying amounts of rabbit immunoglobulin G from 10 pg to 1 mu g. The detection method utilizes an enzyme-amplified immunoassay of 4-methylumbelliferone phosphate as the enzyme substrate. The sensitivity of this assay is three orders of magnitude better than that obtained with a conventional ELISA absorption spectrometric assay using a commercially available reader.

Full Text

Duke Authors

Cited Authors

  • Vo-Dinh, T; Griffin, GD; Ambrose, KR

Published Date

  • January 1, 1986

Published In

Volume / Issue

  • 40 / 5

Start / End Page

  • 696 - 700

International Standard Serial Number (ISSN)

  • 0003-7028

Digital Object Identifier (DOI)

  • 10.1366/0003702864508575

Citation Source

  • Scopus