Skip to main content
Journal cover image

Miniature biochip system for detection of Escherichia coli O157:H7 based on antibody-immobilized capillary reactors and enzyme-linked immunosorbent assay

Publication ,  Journal Article
Song, JM; Vo-Dinh, T
Published in: Analytica Chimica Acta
April 1, 2004

In this work, we report Escherichia coli O157:H7 detection using antibody-immobilized capillary reactors, enzyme-linked immunosorbent assay (ELISA), and a biochip system. ELISA selective immunological method to detect pathogenic bacteria. ELISA is also directly adaptable to a miniature biochip system that utilizes conventional sample platforms such as polymer membranes and glass. The antibody-immobilized capillary reactor is a very attractive sample platform for ELISA because of its low cost, compactness, reuse, and ease of regeneration. Moreover, an array of capillary reactors can provide high-throughput ELISA. In this report, we describe the use of an array of antibody-immobilized capillary reactors for multiplex detection of E. coli O157:H7 in our miniature biochip system. Side-entry laser beam irradiation to an array of capillary reactors contributes significantly to miniaturized optical configuration for this biochip system. The detection limits of E. coli O157:H7 using the ELISA and Cy5 label-based immunoassays were determined to be 3 and 230 cells, respectively. This system shows capability to simultaneously monitor multifunctional immunoassay and high sensitive detection of E. coli O157:H7. © 2003 Elsevier B.V. All rights reserved.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Analytica Chimica Acta

DOI

ISSN

0003-2670

Publication Date

April 1, 2004

Volume

507

Issue

1

Start / End Page

115 / 121

Related Subject Headings

  • Analytical Chemistry
  • 4018 Nanotechnology
  • 4004 Chemical engineering
  • 3401 Analytical chemistry
  • 0399 Other Chemical Sciences
  • 0301 Analytical Chemistry
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Song, J. M., & Vo-Dinh, T. (2004). Miniature biochip system for detection of Escherichia coli O157:H7 based on antibody-immobilized capillary reactors and enzyme-linked immunosorbent assay. Analytica Chimica Acta, 507(1), 115–121. https://doi.org/10.1016/j.aca.2003.11.072
Song, J. M., and T. Vo-Dinh. “Miniature biochip system for detection of Escherichia coli O157:H7 based on antibody-immobilized capillary reactors and enzyme-linked immunosorbent assay.” Analytica Chimica Acta 507, no. 1 (April 1, 2004): 115–21. https://doi.org/10.1016/j.aca.2003.11.072.
Song, J. M., and T. Vo-Dinh. “Miniature biochip system for detection of Escherichia coli O157:H7 based on antibody-immobilized capillary reactors and enzyme-linked immunosorbent assay.” Analytica Chimica Acta, vol. 507, no. 1, Apr. 2004, pp. 115–21. Scopus, doi:10.1016/j.aca.2003.11.072.
Journal cover image

Published In

Analytica Chimica Acta

DOI

ISSN

0003-2670

Publication Date

April 1, 2004

Volume

507

Issue

1

Start / End Page

115 / 121

Related Subject Headings

  • Analytical Chemistry
  • 4018 Nanotechnology
  • 4004 Chemical engineering
  • 3401 Analytical chemistry
  • 0399 Other Chemical Sciences
  • 0301 Analytical Chemistry