Application of a miniature biochip using the molecular beacon probe in breast cancer gene BRCA1 detection.
Publication
, Journal Article
Culha, M; Stokes, DL; Griffin, GD; Vo-Dinh, T
Published in: Biosensors & bioelectronics
April 2004
We report for the first time the application of a biochip using the molecular beacon (MB) detection scheme. The usability of this biochip novel detection system for the analysis of the breast cancer gene BRCA1 is demonstrated using molecular beacon probes. The MB is designed for the BRCA1 gene and a miniature biochip system is used for detection. The performance of the biochip-MB detection system is evaluated. The optimum conditions for the MB system for highest fluorescence detection sensitivity are investigated for the detection system. The detection of BRCA1 gene is successfully demonstrated in solution and the limit of detection (LOD) is estimated as 70 nM.
Duke Scholars
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Published In
Biosensors & bioelectronics
DOI
EISSN
1873-4235
ISSN
0956-5663
Publication Date
April 2004
Volume
19
Issue
9
Start / End Page
1007 / 1012
Related Subject Headings
- Genes, BRCA1
- Fluorescence
- DNA Probes
- Biosensing Techniques
- Bioinformatics
- 4018 Nanotechnology
- 4003 Biomedical engineering
- 3401 Analytical chemistry
- 1007 Nanotechnology
- 0903 Biomedical Engineering
Citation
APA
Chicago
ICMJE
MLA
NLM
Culha, M., Stokes, D. L., Griffin, G. D., & Vo-Dinh, T. (2004). Application of a miniature biochip using the molecular beacon probe in breast cancer gene BRCA1 detection. Biosensors & Bioelectronics, 19(9), 1007–1012. https://doi.org/10.1016/j.bios.2003.09.006
Culha, Mustafa, David L. Stokes, Guy D. Griffin, and Tuan Vo-Dinh. “Application of a miniature biochip using the molecular beacon probe in breast cancer gene BRCA1 detection.” Biosensors & Bioelectronics 19, no. 9 (April 2004): 1007–12. https://doi.org/10.1016/j.bios.2003.09.006.
Culha M, Stokes DL, Griffin GD, Vo-Dinh T. Application of a miniature biochip using the molecular beacon probe in breast cancer gene BRCA1 detection. Biosensors & bioelectronics. 2004 Apr;19(9):1007–12.
Culha, Mustafa, et al. “Application of a miniature biochip using the molecular beacon probe in breast cancer gene BRCA1 detection.” Biosensors & Bioelectronics, vol. 19, no. 9, Apr. 2004, pp. 1007–12. Epmc, doi:10.1016/j.bios.2003.09.006.
Culha M, Stokes DL, Griffin GD, Vo-Dinh T. Application of a miniature biochip using the molecular beacon probe in breast cancer gene BRCA1 detection. Biosensors & bioelectronics. 2004 Apr;19(9):1007–1012.
Published In
Biosensors & bioelectronics
DOI
EISSN
1873-4235
ISSN
0956-5663
Publication Date
April 2004
Volume
19
Issue
9
Start / End Page
1007 / 1012
Related Subject Headings
- Genes, BRCA1
- Fluorescence
- DNA Probes
- Biosensing Techniques
- Bioinformatics
- 4018 Nanotechnology
- 4003 Biomedical engineering
- 3401 Analytical chemistry
- 1007 Nanotechnology
- 0903 Biomedical Engineering