Application of a miniature biochip using the molecular beacon probe in breast cancer gene BRCA1 detection.
Journal Article (Journal Article)
We report for the first time the application of a biochip using the molecular beacon (MB) detection scheme. The usability of this biochip novel detection system for the analysis of the breast cancer gene BRCA1 is demonstrated using molecular beacon probes. The MB is designed for the BRCA1 gene and a miniature biochip system is used for detection. The performance of the biochip-MB detection system is evaluated. The optimum conditions for the MB system for highest fluorescence detection sensitivity are investigated for the detection system. The detection of BRCA1 gene is successfully demonstrated in solution and the limit of detection (LOD) is estimated as 70 nM.
Full Text
Duke Authors
Cited Authors
- Culha, M; Stokes, DL; Griffin, GD; Vo-Dinh, T
Published Date
- April 2004
Published In
Volume / Issue
- 19 / 9
Start / End Page
- 1007 - 1012
PubMed ID
- 15018955
Electronic International Standard Serial Number (EISSN)
- 1873-4235
International Standard Serial Number (ISSN)
- 0956-5663
Digital Object Identifier (DOI)
- 10.1016/j.bios.2003.09.006
Language
- eng