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Application of a miniature biochip using the molecular beacon probe in breast cancer gene BRCA1 detection.

Publication ,  Journal Article
Culha, M; Stokes, DL; Griffin, GD; Vo-Dinh, T
Published in: Biosensors & bioelectronics
April 2004

We report for the first time the application of a biochip using the molecular beacon (MB) detection scheme. The usability of this biochip novel detection system for the analysis of the breast cancer gene BRCA1 is demonstrated using molecular beacon probes. The MB is designed for the BRCA1 gene and a miniature biochip system is used for detection. The performance of the biochip-MB detection system is evaluated. The optimum conditions for the MB system for highest fluorescence detection sensitivity are investigated for the detection system. The detection of BRCA1 gene is successfully demonstrated in solution and the limit of detection (LOD) is estimated as 70 nM.

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Published In

Biosensors & bioelectronics

DOI

EISSN

1873-4235

ISSN

0956-5663

Publication Date

April 2004

Volume

19

Issue

9

Start / End Page

1007 / 1012

Related Subject Headings

  • Genes, BRCA1
  • Fluorescence
  • DNA Probes
  • Biosensing Techniques
  • Bioinformatics
  • 4018 Nanotechnology
  • 4003 Biomedical engineering
  • 3401 Analytical chemistry
  • 1007 Nanotechnology
  • 0903 Biomedical Engineering
 

Citation

APA
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ICMJE
MLA
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Culha, M., Stokes, D. L., Griffin, G. D., & Vo-Dinh, T. (2004). Application of a miniature biochip using the molecular beacon probe in breast cancer gene BRCA1 detection. Biosensors & Bioelectronics, 19(9), 1007–1012. https://doi.org/10.1016/j.bios.2003.09.006
Culha, Mustafa, David L. Stokes, Guy D. Griffin, and Tuan Vo-Dinh. “Application of a miniature biochip using the molecular beacon probe in breast cancer gene BRCA1 detection.Biosensors & Bioelectronics 19, no. 9 (April 2004): 1007–12. https://doi.org/10.1016/j.bios.2003.09.006.
Culha M, Stokes DL, Griffin GD, Vo-Dinh T. Application of a miniature biochip using the molecular beacon probe in breast cancer gene BRCA1 detection. Biosensors & bioelectronics. 2004 Apr;19(9):1007–12.
Culha, Mustafa, et al. “Application of a miniature biochip using the molecular beacon probe in breast cancer gene BRCA1 detection.Biosensors & Bioelectronics, vol. 19, no. 9, Apr. 2004, pp. 1007–12. Epmc, doi:10.1016/j.bios.2003.09.006.
Culha M, Stokes DL, Griffin GD, Vo-Dinh T. Application of a miniature biochip using the molecular beacon probe in breast cancer gene BRCA1 detection. Biosensors & bioelectronics. 2004 Apr;19(9):1007–1012.
Journal cover image

Published In

Biosensors & bioelectronics

DOI

EISSN

1873-4235

ISSN

0956-5663

Publication Date

April 2004

Volume

19

Issue

9

Start / End Page

1007 / 1012

Related Subject Headings

  • Genes, BRCA1
  • Fluorescence
  • DNA Probes
  • Biosensing Techniques
  • Bioinformatics
  • 4018 Nanotechnology
  • 4003 Biomedical engineering
  • 3401 Analytical chemistry
  • 1007 Nanotechnology
  • 0903 Biomedical Engineering