Near-Field Surface-Enhanced Raman Imaging of Dye-Labeled DNA with 100-nm Resolution.

Journal Article (Journal Article)

Raman chemical imaging on a scale of 100 nm is demonstrated for the first time. This is made possible by the combination of scanning near-field optical microscopy (SNOM or NSOM) and surface-enhanced Raman scattering (SERS), using brilliant cresyl blue (BCB)-labeled DNA as a sample. SERS substrates were produced by evaporating silver layers on Teflon nanospheres. The near-field SERS spectra were measured with an exposure time of 60 s and yielded good signal-to-noise ratios (25:1). The distinction between reflected light from the excitation laser and Raman scattered light allows the local sample reflectivity to be separated from the signal of the adsorbed DNA molecules. This is of general importance to correct for topographic coupling that often occurs in near-field optical imaging. The presented data show a lateral dependence of the Raman signals that points to special surface sites with particularly high SERS enhancement.

Full Text

Duke Authors

Cited Authors

  • Deckert, V; Zeisel, D; Zenobi, R; Vo-Dinh, T

Published Date

  • July 1998

Published In

Volume / Issue

  • 70 / 13

Start / End Page

  • 2646 - 2650

PubMed ID

  • 21644784

Electronic International Standard Serial Number (EISSN)

  • 1520-6882

International Standard Serial Number (ISSN)

  • 0003-2700

Digital Object Identifier (DOI)

  • 10.1021/ac971304f


  • eng