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Development of nanosensors and bioprobes

Publication ,  Journal Article
Vo-Dinh, T; Griffin, GD; Alarie, JP; Cullum, B; Sumpter, B; Noid, D
Published in: Journal of Nanoparticle Research
January 1, 2000

We describe the development and application of nanosensors having bioreceptor probes for bioanalysis. The nanoprobes were fabricated with optical fibers pulled down to tips having distal end sizes of approximately 30-60 nm. The use of two different types of receptors was investigated. Fiberoptic nanoprobes were covalently bound either with bioreceptors, such as antibodies, or with other receptors, such as cyclodextrins that are selective for the size and chemical structure of the analyte molecules. Theoretical calculations were performed to model the binding of beta-cyclodextrin with pyrene and 5,6-benzoquinoline, and to illustrate the possibility of comparing experimental data with theoretical data. The antibody-based nanoprobe was used for in situ measurements of benzopyrene tetrol in single cells. The performance of the nanosensor is illustrated by intracellular measurements performed on a rat liver epithelial cell line (Clone 9) used as the model cell system. The usefulness and potential of these nanotechnology-based biosensors in biological research and applications are discussed.

Duke Scholars

Published In

Journal of Nanoparticle Research

DOI

ISSN

1388-0764

Publication Date

January 1, 2000

Volume

2

Issue

1

Start / End Page

17 / 27

Related Subject Headings

  • Nanoscience & Nanotechnology
  • 1007 Nanotechnology
  • 0912 Materials Engineering
  • 0202 Atomic, Molecular, Nuclear, Particle and Plasma Physics
 

Citation

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Vo-Dinh, T., Griffin, G. D., Alarie, J. P., Cullum, B., Sumpter, B., & Noid, D. (2000). Development of nanosensors and bioprobes. Journal of Nanoparticle Research, 2(1), 17–27. https://doi.org/10.1023/A:1010005908586
Vo-Dinh, T., G. D. Griffin, J. P. Alarie, B. Cullum, B. Sumpter, and D. Noid. “Development of nanosensors and bioprobes.” Journal of Nanoparticle Research 2, no. 1 (January 1, 2000): 17–27. https://doi.org/10.1023/A:1010005908586.
Vo-Dinh T, Griffin GD, Alarie JP, Cullum B, Sumpter B, Noid D. Development of nanosensors and bioprobes. Journal of Nanoparticle Research. 2000 Jan 1;2(1):17–27.
Vo-Dinh, T., et al. “Development of nanosensors and bioprobes.” Journal of Nanoparticle Research, vol. 2, no. 1, Jan. 2000, pp. 17–27. Scopus, doi:10.1023/A:1010005908586.
Vo-Dinh T, Griffin GD, Alarie JP, Cullum B, Sumpter B, Noid D. Development of nanosensors and bioprobes. Journal of Nanoparticle Research. 2000 Jan 1;2(1):17–27.
Journal cover image

Published In

Journal of Nanoparticle Research

DOI

ISSN

1388-0764

Publication Date

January 1, 2000

Volume

2

Issue

1

Start / End Page

17 / 27

Related Subject Headings

  • Nanoscience & Nanotechnology
  • 1007 Nanotechnology
  • 0912 Materials Engineering
  • 0202 Atomic, Molecular, Nuclear, Particle and Plasma Physics