Surface-enhanced Raman gene probe for HIV detection.

Journal Article (Journal Article)

We report, for the first time, the use of surface-enhanced Raman (SERS)-active labels for primers used in polymerase chain reaction amplification of specific target DNA sequences. This method has the potential for combining the spectral selectivity and high sensitivity of the SERS technique with the inherent molecular specificity offered by DNA sequence hybridization. The effectiveness of the detection scheme is demonstrated using the gag gene sequence of the human immunodeficiency virus. The potential use of multiple probes for simultaneous detection of multiple biological targets is discussed.

Full Text

Duke Authors

Cited Authors

  • Isola, NR; Stokes, DL; Vo-Dinh, T

Published Date

  • April 1998

Published In

Volume / Issue

  • 70 / 7

Start / End Page

  • 1352 - 1356

PubMed ID

  • 9553492

Electronic International Standard Serial Number (EISSN)

  • 1520-6882

International Standard Serial Number (ISSN)

  • 0003-2700

Digital Object Identifier (DOI)

  • 10.1021/ac970901z


  • eng