Surface-enhanced Raman scattering molecular nanoprobes


Journal Article

Nanoparticles are increasingly finding a wide application in the biological studies due to their unique physical and chemical properties. Colloidal nanoparticles are efficient substrate that exhibit surface-enhanced Raman scattering (SERS) phenomenon by enhancing the scattering cross-sections of conjugated Raman active molecules thus enabling highly sensitive biological probes. However, biological and medical applications would require nanoparticles to be conjugated to biomolecules. A universal approach for conjugation of mercarptoacetic acid-capped silver nanoparticles to biomolecules is described. The surface functionalized silver colloids were labeled with a Raman active dye and used for cellular imaging. We also described the use of silver nanoparticle to develop a new class of SERS nanoprobes for molecular recognition and detection of specific nucleic acid sequences.

Full Text

Duke Authors

Cited Authors

  • Wabuyele, MB; Yan, F; Griffin, GD; Vo-Dinh, T

Published Date

  • July 21, 2005

Published In

Volume / Issue

  • 5692 /

Start / End Page

  • 209 - 215

International Standard Serial Number (ISSN)

  • 1605-7422

Digital Object Identifier (DOI)

  • 10.1117/12.604447

Citation Source

  • Scopus