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Synthesis and characterization of SERS gene probe for BRCA-1 (breast cancer).

Publication ,  Journal Article
Pal, A; Isola, NR; Alarie, JP; Stokes, DL; Vo-Dinh, T
Published in: Faraday discussions
January 2006

A protocol for binding cresyl fast violet (CFV), a SERS-active dye (label) containing an aromatic amino group with a modified oligomer having a carboxy derivatized thymidine moiety using carbodiimide coupling has been achieved for the first time. Covalent coupling between CFV and the oligomer has been confirmed by mass spectral analysis of the labeled oligomer. The fluorescence, SERS and absorption characteristics of the labeled product have been evaluated. The chosen oligomer contains a BRCA-1 (breast cancer) sequence, and hence has the potential for being used as a gene probe to identify BRCA-1 gene. It has high potential for being used in polymerase chain reaction (PCR) amplification, as has been performed with labeled oligonucleotide for the HIV sequence.

Duke Scholars

Published In

Faraday discussions

DOI

EISSN

1364-5498

ISSN

1359-6640

Publication Date

January 2006

Volume

132

Start / End Page

293 / 301

Related Subject Headings

  • Surface Properties
  • Spectrum Analysis, Raman
  • Oxazines
  • Mass Spectrometry
  • Humans
  • Genes, BRCA1
  • Fluorescence
  • Female
  • DNA Probes
  • Chemical Physics
 

Citation

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ICMJE
MLA
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Pal, A., Isola, N. R., Alarie, J. P., Stokes, D. L., & Vo-Dinh, T. (2006). Synthesis and characterization of SERS gene probe for BRCA-1 (breast cancer). Faraday Discussions, 132, 293–301. https://doi.org/10.1039/b506341h
Pal, Anjali, N. R. Isola, J. P. Alarie, D. L. Stokes, and T. Vo-Dinh. “Synthesis and characterization of SERS gene probe for BRCA-1 (breast cancer).Faraday Discussions 132 (January 2006): 293–301. https://doi.org/10.1039/b506341h.
Pal A, Isola NR, Alarie JP, Stokes DL, Vo-Dinh T. Synthesis and characterization of SERS gene probe for BRCA-1 (breast cancer). Faraday discussions. 2006 Jan;132:293–301.
Pal, Anjali, et al. “Synthesis and characterization of SERS gene probe for BRCA-1 (breast cancer).Faraday Discussions, vol. 132, Jan. 2006, pp. 293–301. Epmc, doi:10.1039/b506341h.
Pal A, Isola NR, Alarie JP, Stokes DL, Vo-Dinh T. Synthesis and characterization of SERS gene probe for BRCA-1 (breast cancer). Faraday discussions. 2006 Jan;132:293–301.
Journal cover image

Published In

Faraday discussions

DOI

EISSN

1364-5498

ISSN

1359-6640

Publication Date

January 2006

Volume

132

Start / End Page

293 / 301

Related Subject Headings

  • Surface Properties
  • Spectrum Analysis, Raman
  • Oxazines
  • Mass Spectrometry
  • Humans
  • Genes, BRCA1
  • Fluorescence
  • Female
  • DNA Probes
  • Chemical Physics