A method for quantifying cell size from differential interference contrast images: validation and application to osmotically stressed chondrocytes.


Journal Article

An automatic image analysis method was developed to determine the shape and size of spheroidal cells from a time series of differential interference contrast (DIC) images. The program incorporates an edge detection algorithm and dynamic programming for edge linking. To assess the accuracy and working range of the method, results from DIC images of different focal planes and resolutions were compared to confocal images in which the cell membrane was fluorescently labelled. The results indicate that a 1-microm focal drift from the in-focus plane can lead to an overestimation of cell volume up to 14.1%, mostly due to shadowing effects of DIC microscopy. DIC images allow for accurate measurements when the focal plane lies in a zone slightly above the centre of a spherical cell. In this range the method performs with 1.9% overall volume error without taking into account the error introduced by the representation of the cell as a sphere. As a test case, the method was applied to quantify volume changes due to acute changes of osmotic stress.

Full Text

Cited Authors

  • Alexopoulos, LG; Erickson, GR; Guilak, F

Published Date

  • February 2002

Published In

Volume / Issue

  • 205 / Pt 2

Start / End Page

  • 125 - 135

PubMed ID

  • 11879427

Pubmed Central ID

  • 11879427

Electronic International Standard Serial Number (EISSN)

  • 1365-2818

International Standard Serial Number (ISSN)

  • 0022-2720

Digital Object Identifier (DOI)

  • 10.1046/j.0022-2720.2001.00976.x


  • eng