Inhibition of implant-associated infections via nitric oxide release.
Journal Article (Journal Article)
The in vivo antibacterial activity of nitric oxide (NO)-releasing xerogel coatings was evaluated against an aggressive subcutaneous Staphylococcus aureus infection in a rat model. The NO-releasing implants were created by coating a medical-grade silicone elastomer with a sol-gel-derived (xerogel) film capable of storing NO. Four of the bare or xerogel-coated silicone materials were subcutaneously implanted into male rats. Ten rats were administered 10 microl of a 10(8) cfuml(-1)S. aureus colony directly into the subcutaneous pocket with the implant prior to wound closure. Infection was quantitatively and qualitatively evaluated after 8d of implantation with microbiological and histological methods, respectively. A 82% reduction in the number of infected implants was achieved with the NO-releasing coating. Histology revealed that the capsule formation around infected bare silicone rubber controls was immunoactive and that a biofilm may have formed. Capsule formation in response to NO-releasing implants had greater vascularity in comparison with uninoculated or untreated controls. These results suggest that NO-releasing coatings may dramatically reduce the incidence of biomaterial-associated infection.
Full Text
Duke Authors
Cited Authors
- Nablo, BJ; Prichard, HL; Butler, RD; Klitzman, B; Schoenfisch, MH
Published Date
- December 2005
Published In
Volume / Issue
- 26 / 34
Start / End Page
- 6984 - 6990
PubMed ID
- 15978663
International Standard Serial Number (ISSN)
- 0142-9612
Digital Object Identifier (DOI)
- 10.1016/j.biomaterials.2005.05.017
Language
- eng
Conference Location
- Netherlands