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Synthesis of radioiodinated N-succinimidyl iodobenzoate: optimization for use in antibody labelling.

Publication ,  Journal Article
Garg, PK; Archer, GE; Bigner, DD; Zalutsky, MR
Published in: Int J Rad Appl Instrum A
1989

N-succinimidyl-3-(tri-n-butylstannyl)benzoate (m-BuATE), N-succinimidyl-3-(tri-methylstannyl)benzoate (m-MeATE) and N-succinimidyl-4-(tri-n-butylstannyl)benzoate (p-BuATE) were synthesized and radioiodinated using either N-chlorosuccinimide (NCS) or t-butylhydroperoxide (TBHP) as the oxidant. Radiohalogenation of m-MeATE proceeded more rapidly than m-BuATE. NCS was the more efficient oxidant at reaction times less than 15 min; use of both TBHP and NCS resulted in nearly quantitative yields after 15 min when m-MeATE was used. Using NCS, achieving optimal antibody coupling and specific binding required purification of the active ester by HPLC; in contrast, with TBHP, only Sep-Pak purification was needed.

Duke Scholars

Published In

Int J Rad Appl Instrum A

DOI

ISSN

0883-2889

Publication Date

1989

Volume

40

Issue

6

Start / End Page

485 / 490

Location

England

Related Subject Headings

  • Trimethyltin Compounds
  • Trialkyltin Compounds
  • Isotope Labeling
  • Iodine Radioisotopes
  • Brain Neoplasms
  • Benzoates
  • Antibodies, Neoplasm
 

Citation

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Garg, P. K., Archer, G. E., Bigner, D. D., & Zalutsky, M. R. (1989). Synthesis of radioiodinated N-succinimidyl iodobenzoate: optimization for use in antibody labelling. Int J Rad Appl Instrum A, 40(6), 485–490. https://doi.org/10.1016/0883-2889(89)90131-7
Garg, P. K., G. E. Archer, D. D. Bigner, and M. R. Zalutsky. “Synthesis of radioiodinated N-succinimidyl iodobenzoate: optimization for use in antibody labelling.Int J Rad Appl Instrum A 40, no. 6 (1989): 485–90. https://doi.org/10.1016/0883-2889(89)90131-7.
Garg PK, Archer GE, Bigner DD, Zalutsky MR. Synthesis of radioiodinated N-succinimidyl iodobenzoate: optimization for use in antibody labelling. Int J Rad Appl Instrum A. 1989;40(6):485–90.
Garg, P. K., et al. “Synthesis of radioiodinated N-succinimidyl iodobenzoate: optimization for use in antibody labelling.Int J Rad Appl Instrum A, vol. 40, no. 6, 1989, pp. 485–90. Pubmed, doi:10.1016/0883-2889(89)90131-7.
Garg PK, Archer GE, Bigner DD, Zalutsky MR. Synthesis of radioiodinated N-succinimidyl iodobenzoate: optimization for use in antibody labelling. Int J Rad Appl Instrum A. 1989;40(6):485–490.

Published In

Int J Rad Appl Instrum A

DOI

ISSN

0883-2889

Publication Date

1989

Volume

40

Issue

6

Start / End Page

485 / 490

Location

England

Related Subject Headings

  • Trimethyltin Compounds
  • Trialkyltin Compounds
  • Isotope Labeling
  • Iodine Radioisotopes
  • Brain Neoplasms
  • Benzoates
  • Antibodies, Neoplasm