Skip to main content
Journal cover image

Retinoic acid-mediated gene expression in transgenic reporter zebrafish.

Publication ,  Journal Article
Perz-Edwards, A; Hardison, NL; Linney, E
Published in: Dev Biol
January 1, 2001

Retinoic acid-mediated gene activation is important for normal vertebrate development. The size and nature of retinoic acid make it difficult to identify the precise cellular location of this signaling molecule throughout an embryo. Additionally, retinoic acid (RA) signaling is regulated by a complex combination of receptors, coactivators, and antagonizing proteins. Thus, in order to integrate these signals and identify regions within a whole developing embryo where cells can respond transcriptionally to retinoic acid, we have used a reporter transgenic approach. We have generated several stable lines of transgenic zebrafish which use retinoic acid response elements to drive fluorescent protein expression. In these zebrafish lines, transgene expression is localized to regions of the neural tube, retina, notochord, somites, heart, pronephric ducts, branchial arches, and jaw muscles in embryos and larvae. Transgene expression can be induced in additional regions of the neural tube and retina as well as the immature notochord, hatching gland, enveloping cell layer, and fin by exposing embryos to retinoic acid. Treatment with retinoic acid synthase inhibitors, citral and diethylaminobenzaldehyde (DEAB), during neurulation, greatly reduces transgene expression. DEAB treatment of embryos at gastrulation phenocopies the embryonic effects of vitamin A deprivation or targeted disruption of the RA synthase retinaldehyde dehydrogenase-2 in other vertebrates. Together these data suggest that the reporter expression we see in zebrafish is dependent upon conserved vertebrate pathways of RA synthesis.

Duke Scholars

Published In

Dev Biol

DOI

ISSN

0012-1606

Publication Date

January 1, 2001

Volume

229

Issue

1

Start / End Page

89 / 101

Location

United States

Related Subject Headings

  • Zebrafish Proteins
  • Zebrafish
  • Vitamin A Deficiency
  • Tretinoin
  • Transgenes
  • Transcriptional Activation
  • Terpenes
  • Teratogens
  • Retinal Dehydrogenase
  • Promoter Regions, Genetic
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Perz-Edwards, A., Hardison, N. L., & Linney, E. (2001). Retinoic acid-mediated gene expression in transgenic reporter zebrafish. Dev Biol, 229(1), 89–101. https://doi.org/10.1006/dbio.2000.9979
Perz-Edwards, A., N. L. Hardison, and E. Linney. “Retinoic acid-mediated gene expression in transgenic reporter zebrafish.Dev Biol 229, no. 1 (January 1, 2001): 89–101. https://doi.org/10.1006/dbio.2000.9979.
Perz-Edwards A, Hardison NL, Linney E. Retinoic acid-mediated gene expression in transgenic reporter zebrafish. Dev Biol. 2001 Jan 1;229(1):89–101.
Perz-Edwards, A., et al. “Retinoic acid-mediated gene expression in transgenic reporter zebrafish.Dev Biol, vol. 229, no. 1, Jan. 2001, pp. 89–101. Pubmed, doi:10.1006/dbio.2000.9979.
Perz-Edwards A, Hardison NL, Linney E. Retinoic acid-mediated gene expression in transgenic reporter zebrafish. Dev Biol. 2001 Jan 1;229(1):89–101.
Journal cover image

Published In

Dev Biol

DOI

ISSN

0012-1606

Publication Date

January 1, 2001

Volume

229

Issue

1

Start / End Page

89 / 101

Location

United States

Related Subject Headings

  • Zebrafish Proteins
  • Zebrafish
  • Vitamin A Deficiency
  • Tretinoin
  • Transgenes
  • Transcriptional Activation
  • Terpenes
  • Teratogens
  • Retinal Dehydrogenase
  • Promoter Regions, Genetic