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Global expression analysis of cancer/testis genes in uterine cancers reveals a high incidence of BORIS expression.

Publication ,  Journal Article
Risinger, JI; Chandramouli, GVR; Maxwell, GL; Custer, M; Pack, S; Loukinov, D; Aprelikova, O; Litzi, T; Schrump, DS; Murphy, SK; Berchuck, A ...
Published in: Clin Cancer Res
March 15, 2007

PURPOSE: Cancer/testis (CT) genes predominantly expressed in the testis (germ cells) and generally not in other normal tissues are aberrantly expressed in human cancers. This highly restricted expression provides a unique opportunity to use these CT genes for diagnostics, immunotherapeutic, or other targeted therapies. The purpose of this study was to identify those CT genes with the greatest incidence of expression in uterine cancers. EXPERIMENTAL DESIGN: We queried the expression of known and putative CT gene transcripts (representing 79 gene loci) using whole genome gene expression arrays. Specifically, the global gene expressions of uterine cancers (n = 122) and normal uteri (n = 10) were determined using expression data from the Affymetrix HG-U133A and HG-U133B chips. Additionally, we also examined the brother of the regulator of imprinted sites (BORIS) transcript by reverse transcription-PCR and quantitative PCR because its transcript was not represented on the array. RESULTS: Global microarray analysis detected many CT genes expressed in various uterine cancers; however, no individual CT gene was expressed in more than 25% of all cancers. The expression of the two most commonly expressed CT genes on the arrays, MAGEA9 (24 of 122 cancers and 0 of 10 normal tissues) and Down syndrome critical region 8 (DSCR8)/MMA1 (16 if 122 cancers and 0 of 10 normal tissues), was confirmed by reverse transcription-PCR methods, validating the array screening approach. In contrast to the relatively low incidence of expression of the other CT genes, BORIS expression was detected in 73 of 95 (77%) endometrial cancers and 24 of 31 (77%) uterine mixed mesodermal tumors. CONCLUSIONS: These data provide the first extensive survey of multiple CT genes in uterine cancers. Importantly, we detected a high frequency of BORIS expression in uterine cancers, suggesting its potential as an immunologic or diagnostic target for these cancers. Given the high incidence of BORIS expression and its possible regulatory role, an examination of BORIS function in the etiology of these cancers is warranted.

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Published In

Clin Cancer Res

DOI

ISSN

1078-0432

Publication Date

March 15, 2007

Volume

13

Issue

6

Start / End Page

1713 / 1719

Location

United States

Related Subject Headings

  • Uterine Neoplasms
  • Testis
  • Oncology & Carcinogenesis
  • Oligonucleotide Array Sequence Analysis
  • Neoplasm Proteins
  • Male
  • Humans
  • Genes, Neoplasm
  • Gene Expression Regulation, Neoplastic
  • Female
 

Citation

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Risinger, J. I., Chandramouli, G. V. R., Maxwell, G. L., Custer, M., Pack, S., Loukinov, D., … Barrett, J. C. (2007). Global expression analysis of cancer/testis genes in uterine cancers reveals a high incidence of BORIS expression. Clin Cancer Res, 13(6), 1713–1719. https://doi.org/10.1158/1078-0432.CCR-05-2569
Risinger, John Ian, Gadisetti V. R. Chandramouli, G Larry Maxwell, Mary Custer, Svetlana Pack, Dmitri Loukinov, Olga Aprelikova, et al. “Global expression analysis of cancer/testis genes in uterine cancers reveals a high incidence of BORIS expression.Clin Cancer Res 13, no. 6 (March 15, 2007): 1713–19. https://doi.org/10.1158/1078-0432.CCR-05-2569.
Risinger JI, Chandramouli GVR, Maxwell GL, Custer M, Pack S, Loukinov D, et al. Global expression analysis of cancer/testis genes in uterine cancers reveals a high incidence of BORIS expression. Clin Cancer Res. 2007 Mar 15;13(6):1713–9.
Risinger, John Ian, et al. “Global expression analysis of cancer/testis genes in uterine cancers reveals a high incidence of BORIS expression.Clin Cancer Res, vol. 13, no. 6, Mar. 2007, pp. 1713–19. Pubmed, doi:10.1158/1078-0432.CCR-05-2569.
Risinger JI, Chandramouli GVR, Maxwell GL, Custer M, Pack S, Loukinov D, Aprelikova O, Litzi T, Schrump DS, Murphy SK, Berchuck A, Lobanenkov V, Barrett JC. Global expression analysis of cancer/testis genes in uterine cancers reveals a high incidence of BORIS expression. Clin Cancer Res. 2007 Mar 15;13(6):1713–1719.

Published In

Clin Cancer Res

DOI

ISSN

1078-0432

Publication Date

March 15, 2007

Volume

13

Issue

6

Start / End Page

1713 / 1719

Location

United States

Related Subject Headings

  • Uterine Neoplasms
  • Testis
  • Oncology & Carcinogenesis
  • Oligonucleotide Array Sequence Analysis
  • Neoplasm Proteins
  • Male
  • Humans
  • Genes, Neoplasm
  • Gene Expression Regulation, Neoplastic
  • Female