Whipple's arthritis: direct detection of Tropheryma whippelii in synovial fluid and tissue.

Published

Journal Article

We describe 2 patients presenting with polyarthritis in whom the synovial fluid (1 patient) or synovial tissue (1 patient) was positive for Tropheryma whippelii, the Whipple's disease-associated bacillus, when examined by polymerase chain reaction (PCR) and DNA sequencing. Histopathologic findings were consistent with articular Whipple's disease in the synovial fluid of 1 patient and the synovial tissue of the other. In both patients, bowel mucosal specimens were negative for Whipple's disease features by histologic and PCR methods. One patient was positive for T whippelii in the peripheral blood. Control synovial fluid specimens from 40 patients with other arthritides, including Lyme arthritis, were negative. Sequencing of a 284-basepair region of the 16S ribosomal RNA gene confirmed that the sequence is closely related to the known T whippelii sequence. Both patients responded to treatment with antibiotics.

Full Text

Duke Authors

Cited Authors

  • O'Duffy, JD; Griffing, WL; Li, CY; Abdelmalek, MF; Persing, DH

Published Date

  • April 1999

Published In

Volume / Issue

  • 42 / 4

Start / End Page

  • 812 - 817

PubMed ID

  • 10211899

Pubmed Central ID

  • 10211899

International Standard Serial Number (ISSN)

  • 0004-3591

Digital Object Identifier (DOI)

  • 10.1002/1529-0131(199904)42:4<812::AID-ANR27>3.0.CO;2-S

Language

  • eng

Conference Location

  • United States