Increased surfactant protein D in rat airway goblet and Clara cells during ovalbumin-induced allergic airway inflammation.


Journal Article

BACKGROUND:Structural remodelling of airways in asthma that follows inflammation may be affected by surfactant protein D (SP-D)-mediated effects on the immune response. OBJECTIVE:To determine potential sites of SP-D interaction with the pulmonary immune response, we examined the distribution of immunoreactive SP-D in an experimental model of allergen-induced airway inflammation using immunohistochemistry, biochemical methods and in situ hybridization. METHODS:The experimental model used subcutaneous injection of ovalbumin in adult rats, which induced an airway response to inhaled nebulized ovalbumin. Three groups of rats (ovalbumin, ovalbumin + dexamethasone and saline) were challenged thrice weekly for 3 weeks. A fourth group of seven rats (naive) were taken from the same delivery of rats as the other groups. Lungs were then lavaged to determine total cell count, eosinophil count, ovalbumin-specific IgE by enzyme-linked immunosorbent assay and SP-D by immunoblot. Tissue samples were fixed and embedded, and sections were studied for the infiltration of eosinophils and for expression of SP-D protein by histochemistry and mRNA by in situ hybridization. RESULTS:Ovalbumin induced perivascular and peribronchiolar eosinophilia which could be prevented by dexamethasone treatment. In addition, the ovalbumin-specific IgE levels in serum and bronchoalveolar lavage fluid of ovalbumin-challenged animals were enhanced. Increased amount of SP-D in lavage and tissue, particularly in type II pneumocytes, in Clara cells and, surprisingly, in hyperplastic goblet cells of inflamed lungs was found. SP-D mRNA was detected in goblet cells as well as in type II pneumocytes and Clara cells. Dexamethasone treatment did not affect level of SP-D immunoreactivity. CONCLUSION:SP-D accumulation is increased in this model of allergen-induced eosinophilia, both in upper and lower airways. The increase is unaffected by dexamethasone.

Full Text

Cited Authors

  • Kasper, M; Sims, G; Koslowski, R; Kuss, H; Thuemmler, M; Fehrenbach, H; Auten, RL

Published Date

  • August 2002

Published In

Volume / Issue

  • 32 / 8

Start / End Page

  • 1251 - 1258

PubMed ID

  • 12190667

Pubmed Central ID

  • 12190667

Electronic International Standard Serial Number (EISSN)

  • 1365-2222

International Standard Serial Number (ISSN)

  • 0954-7894

Digital Object Identifier (DOI)

  • 10.1046/j.1365-2745.2002.01423.x


  • eng