Extracellular superoxide dismutase protects lung development in hyperoxia-exposed newborn mice.
Journal Article (Journal Article)
We tested the hypothesis that targeted transgenic overexpression of human extracellular superoxide dismutase (EC-SOD) would preserve alveolar development in hyperoxia-exposed newborn mice. We exposed newborn transgenic and wild-type mice to 95% oxygen (O2) or air x 7 days and measured bronchoalveolar lavage cell counts, and lung homogenate EC-SOD, oxidized and reduced glutathione, and myeloperoxidase. We found that total EC-SOD activity in transgenic newborn mice was approximately 2.5x the wild-type activity. Hyperoxia-exposed transgenic mice had less pulmonary neutrophil influx and oxidized glutathione than wild-type littermates at 7 days. We measured alveolar surface and volume density in animals exposed to 14 days more of air or 60% O2. Hyperoxia-exposed transgenic EC-SOD mice had significant preservation of alveolar surface and volume density compared with wild-type littermates. After 7 days 95% O2 + 14 days 60% O2, lung inflammation measured as myeloperoxidase activity was reduced to control levels in all treatment groups.
Full Text
Duke Authors
Cited Authors
- Ahmed, MN; Suliman, HB; Folz, RJ; Nozik-Grayck, E; Golson, ML; Mason, SN; Auten, RL
Published Date
- February 1, 2003
Published In
Volume / Issue
- 167 / 3
Start / End Page
- 400 - 405
PubMed ID
- 12406846
International Standard Serial Number (ISSN)
- 1073-449X
Digital Object Identifier (DOI)
- 10.1164/rccm.200202-108OC
Language
- eng
Conference Location
- United States