Extracellular superoxide dismutase protects lung development in hyperoxia-exposed newborn mice.

Journal Article (Journal Article)

We tested the hypothesis that targeted transgenic overexpression of human extracellular superoxide dismutase (EC-SOD) would preserve alveolar development in hyperoxia-exposed newborn mice. We exposed newborn transgenic and wild-type mice to 95% oxygen (O2) or air x 7 days and measured bronchoalveolar lavage cell counts, and lung homogenate EC-SOD, oxidized and reduced glutathione, and myeloperoxidase. We found that total EC-SOD activity in transgenic newborn mice was approximately 2.5x the wild-type activity. Hyperoxia-exposed transgenic mice had less pulmonary neutrophil influx and oxidized glutathione than wild-type littermates at 7 days. We measured alveolar surface and volume density in animals exposed to 14 days more of air or 60% O2. Hyperoxia-exposed transgenic EC-SOD mice had significant preservation of alveolar surface and volume density compared with wild-type littermates. After 7 days 95% O2 + 14 days 60% O2, lung inflammation measured as myeloperoxidase activity was reduced to control levels in all treatment groups.

Full Text

Duke Authors

Cited Authors

  • Ahmed, MN; Suliman, HB; Folz, RJ; Nozik-Grayck, E; Golson, ML; Mason, SN; Auten, RL

Published Date

  • February 1, 2003

Published In

Volume / Issue

  • 167 / 3

Start / End Page

  • 400 - 405

PubMed ID

  • 12406846

International Standard Serial Number (ISSN)

  • 1073-449X

Digital Object Identifier (DOI)

  • 10.1164/rccm.200202-108OC


  • eng

Conference Location

  • United States