Structural analysis of an HLA-B7 antigen variant detected by cytotoxic T lymphocytes.

Journal Article (Journal Article)

It has been demonstrated previously that lymphocytes of donor CF (HLA-A29,w33; B7,14) are not recognized by the HLA-B7-specific CTL clone HG-31. This report presents a structural comparison of the HLA-B7 antigen of donor CF with a "normal" HLA-B7 antigen, derived from the cell line JY. Isoelectric focusing showed that CF HLA-B7 heavy chains were more acidic than JY HLA-B7 heavy chains by the equivalent of a single charge. High pressure liquid chromatography and ion exchange chromatography comparisons of double-labeled tryptic peptides revealed a single detectable difference, which corresponded to the tryptic peptide spanning residues 112 to 121 on the HLA-B7 heavy chain. Although the complete amino acid sequence of this peptide was not obtained, the partial sequence indicates a substitution of an unidentified amino acid for tyrosine at position 116 of the heavy chain. This residue is found to vary among HLA specificities and to be altered in many H-2Kb mutants.

Full Text

Duke Authors

Cited Authors

  • Taketani, S; Krangel, MS; Spits, H; de Vries, J; Strominger, JL

Published Date

  • August 1, 1984

Published In

Volume / Issue

  • 133 / 2

Start / End Page

  • 816 - 821

PubMed ID

  • 6429245

International Standard Serial Number (ISSN)

  • 0022-1767


  • eng

Conference Location

  • United States